Comparative Structural Investigation of Histone-Like HU Proteins by Small-Angle X-ray Scattering
- Autores: Petoukhov M.1,2,3, Rakitina T.4,5, Agapova Y.4, Petrenko D.4, Konarev P.1,4, Britikov V.6, Britikova E.6, Bocharov E.2, Shtykova E.1
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Afiliações:
- Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
- Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119071, Moscow, Russia
- National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia
- Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia
- Institute of Bioorganic Chemistry, National Academy of Science of Belarus, Minsk, Belarus
- Edição: Volume 68, Nº 6 (2023)
- Páginas: 914-921
- Seção: STRUCTURE OF MACROMOLECULAR COMPOUNDS
- URL: https://journals.rcsi.science/0023-4761/article/view/231829
- DOI: https://doi.org/10.31857/S0023476123600143
- EDN: https://elibrary.ru/FZWVGJ
- ID: 231829
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Resumo
Nucleoid-associated proteins (NAPs) control the structure and functions of bacterial nucleoid. Histone-like HU proteins are most abundant NAPs in dividing bacterial cells. Previously, structural ensembles of conformations of HU proteins from pathogenic mycoplasmas Spiroplasma melliferum and Mycoplasma gallisepticum were obtained using NMR spectroscopy. A structural study of these mycoplasma proteins is performed by small-angle X-ray scattering (SAXS). The occurrence of individual conformations from the ensemble, obtained by NMR, is estimated from the scattering data on HU protein solutions. In particular, an approach based on characterization of equilibrium mixtures in terms of volume fractions of their components was applied. The general shape of the proteins and their oligomeric state are independently confirmed using ab initio bead modelling. The flexibility of DNA-binding protein domains is analyzed by the ensemble optimization method, which is based on comparison of the structural characteristics of conformations fitting the SAXS data to the distribution of these characteristics in a randomly generated set. The results obtained give a new insight on the variability of the structure of HU proteins, which is necessary for their functioning.
Sobre autores
M. Petoukhov
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia; Frumkin Institute of Physical Chemistry and Electrochemistry, Russian Academy of Sciences, 119071, Moscow, Russia
Email: pmxmvl@yandex.ru
Россия, Москва; Россия, Москва; Россия, Москва
T. Rakitina
National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia; Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia
Email: pmxmvl@yandex.ru
Россия, Москва; Россия, Москва
Yu. Agapova
National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia
Email: pmxmvl@yandex.ru
Россия, Москва
D. Petrenko
National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia
Email: pmxmvl@yandex.ru
Россия, Москва
P. Konarev
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia; National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia
Email: pmxmvl@yandex.ru
Россия, Москва; Россия, Москва
V. Britikov
Institute of Bioorganic Chemistry, National Academy of Science of Belarus, Minsk, Belarus
Email: pmxmvl@yandex.ru
Беларусь, Минск
E. Britikova
Institute of Bioorganic Chemistry, National Academy of Science of Belarus, Minsk, Belarus
Email: pmxmvl@yandex.ru
Беларусь, Минск
E. Bocharov
Shemyakin–Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Moscow, Russia
Email: pmxmvl@yandex.ru
Россия, Москва
E. Shtykova
Shubnikov Institute of Crystallography, Federal Scientific Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia
Autor responsável pela correspondência
Email: pmxmvl@yandex.ru
Россия, Москва
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