Effect of Reversion Back to Cys11 on the Structure and Function of S11C Cys-free Nt.BspD6I
- Autores: Artyukh R.1, Fatkhullin B.2, Antipova V.1, Perevyazova T.1, Kachalova G.1, Yunusova A.1
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Afiliações:
- Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
- Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
- Edição: Volume 68, Nº 6 (2023)
- Páginas: 859-865
- Seção: STRUCTURE OF MACROMOLECULAR COMPOUNDS
- URL: https://journals.rcsi.science/0023-4761/article/view/231802
- DOI: https://doi.org/10.31857/S0023476123600702
- EDN: https://elibrary.ru/YEJZJU
- ID: 231802
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Resumo
The three-dimensional structure of recombinant nicking endonuclease S11C Cys-free Nt.BspD6I was determined at 1.85 Å resolution. Nickase S11C Cys-free Nt.BspD6I was produced by the reversion back to Cys11 in Cys-free Nt.BspD6I using site-directed mutagenesis. An analysis of the crystal structure of nickase S11C Cys-free Nt.BspD6I demonstrated that the reversion back to Cys11 induces significant conformational changes in the recognition domain of nickase, which are accompanied by changes in its functions, such as a decrease in the affinity to DNA, the loss of the ability to undergo oligomerization, and high activity of restriction endonuclease S11C Cys-free R.BspD6I.
Sobre autores
R. Artyukh
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
Email: rimmaartyukh@gmail.com
Россия, Пущино
B. Fatkhullin
Institute of Protein Research, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
Email: rimmaartyukh@gmail.com
Россия, Пущино
V. Antipova
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
Email: rimmaartyukh@gmail.com
Россия, Пущино
T. Perevyazova
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
Email: rimmaartyukh@gmail.com
Россия, Пущино
G. Kachalova
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
Email: rimmaartyukh@gmail.com
Россия, Пущино
A. Yunusova
Institute of Theoretical and Experimental Biophysics, Russian Academy of Sciences, 142290, Pushchino, Moscow oblast, Russia
Autor responsável pela correspondência
Email: rimmaartyukh@gmail.com
Россия, Пущино
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