电邮这篇文章 Preliminary X-ray Study of Crystals Obtained by Co-Crystallization of Hypoxanthine‒Guanine Phosphoribosyltransferase from Escherichia coli and Pyrazine-2-Carboxamide Derivatives
- 作者: Abramchik Y.1,2, Zayats E.1, Timofeev V.2,3, Shevtsov M.4, Kostromina M.1, Fateev I.1, Yurovskaya D.1, Karanov A.1, Konstantinova I.1, Kuranova I.2,3, Esipov R.1
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隶属关系:
- Shemyakin‒Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia
- Shubnikov Institute of Crystallography, Federal Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia
- National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia
- Moscow Institute of Physics and Technology, 141700, Dolgoprudnyi, Moscow oblast, Russia
- 期: 卷 68, 编号 6 (2023)
- 页面: 854-858
- 栏目: STRUCTURE OF MACROMOLECULAR COMPOUNDS
- URL: https://journals.rcsi.science/0023-4761/article/view/231798
- DOI: https://doi.org/10.31857/S002347612360012X
- EDN: https://elibrary.ru/ZZVSQF
- ID: 231798
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详细
A highly effective producer strain Escherichia coli C3030/pET23d+-EcHGPRT, allowing production of recombinant hypoxanthine‒guanine phosphoribosyltransferase from E. coli (EcHGPRT) in a soluble form, has been created. A method for isolating and purifying the recombinant protein has been developed. The specific activity against the natural substrate and pyrazine-2-carboxamide derivatives has been determined. Crystals of the EcHGPRT complexes with 3-hydroxypyrazine-2-carboxamide (T-1105) and 6-fluoro-3-hydroxypyrazine-2-carboxamide (T-705), suitable for X-ray diffraction analysis, have been grown by capillary counter diffusion. X-ray diffraction sets with a resolution of up to 2.4 and 2.5 Å have been collected at the ESRF synchrotron (France, station ID23-1) at a temperature of 100 K. The crystals belong to the sp. gr. P3(1)21; the independent part of the cell contains two enzyme molecules.
作者简介
Yu. Abramchik
Shemyakin‒Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia; Shubnikov Institute of Crystallography, Federal Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia
Email: ugama@yandex.ru
Россия, Москва; Россия, Москва
E. Zayats
Shemyakin‒Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia
Email: ugama@yandex.ru
Россия, Москва
V. Timofeev
Shubnikov Institute of Crystallography, Federal Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia; National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia
Email: ugama@yandex.ru
Россия, Москва; Россия, Москва
M. Shevtsov
Moscow Institute of Physics and Technology, 141700, Dolgoprudnyi, Moscow oblast, Russia
Email: ugama@yandex.ru
Россия, Долгопрудный
M. Kostromina
Shemyakin‒Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia
Email: ugama@yandex.ru
Россия, Москва
I. Fateev
Shemyakin‒Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia
Email: ugama@yandex.ru
Россия, Москва
D. Yurovskaya
Shemyakin‒Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia
Email: ugama@yandex.ru
Россия, Москва
A. Karanov
Shemyakin‒Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia
Email: ugama@yandex.ru
Россия, Москва
I. Konstantinova
Shemyakin‒Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia
Email: ugama@yandex.ru
Россия, Москва
I. Kuranova
Shubnikov Institute of Crystallography, Federal Research Centre “Crystallography and Photonics,” Russian Academy of Sciences, 119333, Moscow, Russia; National Research Centre “Kurchatov Institute”, 123182, Moscow, Russia
Email: ugama@yandex.ru
Россия, Москва; Россия, Москва
R. Esipov
Shemyakin‒Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, 117997, Moscow, Russia
编辑信件的主要联系方式.
Email: ugama@yandex.ru
Россия, Москва
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