Selection of the optimal concentration of sodium chloride (NaCl) solution for rapid assessment of cucumber (Cucumis sativus L.) for salinity resistance

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Abstract

The article presents the results of research on the selection of the optimal concentration of sodium chloride solution (NaCl) for the evaluation of the source material and the search for genetic sources of resistance to salinity for cucumber breeding (Cucumis sativus L.). The research was conducted at ARRIVG, a branch of the Federal State Budgetary Scientific and Scientific Institution of the Russian Federation and Poisk Agrofirm LLC (Russian Federation, Moscow Region, Ramenskiy district, Vereya village) during 2021-2023. The laboratory experiment was carried out in two versions: with dry and sprouted cucumber seeds. Based on an analysis of the effect of the concentration of sodium chloride solutions (NaCl) (50, 100, 150, 200, 250, 300 mmol/l) has an inhibitory effect on cucumber seedlings (a decrease in the number of germinated seeds in a solution of sodium chloride (NaCl), a decrease in the length of the root and the average weight of one seedling). The optimal concentration of 200 mmol/l sodium chloride (NaCl) solution was determined to assess the development of cucumber seedlings. The effectiveness of using this selection material assessment method has been proven. A strong negative inverse effect of sodium chloride (NaCl) solution concentration on cucumber seed germination, rootlet length, and average sprout weight (r> –0,80) was revealed. Strong positive direct relationships were found between the number of germinated seeds in sodium chloride (NaCl) solution and rootlet length (r> 0,70); average sprout weight and rootlet length (r> 0,90); average sprout weight (sprouted seeds) and average sprout weight (dry seeds) (r> 0,90); rootlet length (sprouted seeds) and rootlet length (dry seeds) (r> 0,90).

About the authors

L. A Chistyakova

ARRIVG – branch of FSBSI FSCV

A. A Egorova

ARRIVG – branch of FSBSI FSCV

O. V Baklanova

ARRIVG – branch of FSBSI FSCV

A. N Khovrin

ARRIVG – branch of FSBSI FSCV

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