Comparative analysis of immune responses in Colorado potato beetle larvae during development of mycoses caused by Metarhizium robertsii, M. brunneum, and M. pemphigi
- 作者: Tyurin M.1, Kryukov V.1, Yaroslavtseva O.1, Elisafenko E.2, Dubovskiy I.1, Glupov V.1
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隶属关系:
- Institute of Systematics and Ecology of Animals
- Institute of Cytology and Genetics
- 期: 卷 52, 编号 3 (2016)
- 页面: 252-260
- 栏目: Comparative and Ontogenic Physiology
- URL: https://journals.rcsi.science/0022-0930/article/view/159052
- DOI: https://doi.org/10.1134/S002209301603008X
- ID: 159052
如何引用文章
详细
Development of mycoses and progress of humoral and cellular immune responses were compared in larvae of the Colorado potato beetle Leptinotarsa decemlineata infected with entomopathogenic fungi Metarhizium robertsii, M. brunneum and M. pemphigi. The larvae were found to be highly susceptible to the strains of M. robertsii and M. brunneum but weakly responsive to M. pemphigi. The extent of susceptibility to the pathogens was not related to the stimulating effect of epicuticular extracts on fungal growth. Metarhizium pemphigi, which is non-specific to the Colorado potato beetle, did not cause any significant changes in the immune response and did not colonize the hemocoel. When infected with M. robertsii and M. brunneum, the larvae exhibited an increase in hemocyte count during the early stage of mycosis (day 2) followed by a drastic decrease on day 3. The immunocompetent cells, plasmatocytes and granulocytes, exhibited the greatest decrease. Elevated phenoloxidase activity was recorded in the hemolymph and cuticle on days 2 and 3 post-infection. These changes in the immune responses correlated with strain-specific virulence. Thus, the immune response in Colorado potato beetle larvae is an important factor, which determines differences in the development of mycoses caused by different Metarhizium species.
作者简介
M. Tyurin
Institute of Systematics and Ecology of Animals
Email: krukoff@mail.ru
俄罗斯联邦, Novosibirsk
V. Kryukov
Institute of Systematics and Ecology of Animals
编辑信件的主要联系方式.
Email: krukoff@mail.ru
俄罗斯联邦, Novosibirsk
O. Yaroslavtseva
Institute of Systematics and Ecology of Animals
Email: krukoff@mail.ru
俄罗斯联邦, Novosibirsk
E. Elisafenko
Institute of Cytology and Genetics
Email: krukoff@mail.ru
俄罗斯联邦, Novosibirsk
I. Dubovskiy
Institute of Systematics and Ecology of Animals
Email: krukoff@mail.ru
俄罗斯联邦, Novosibirsk
V. Glupov
Institute of Systematics and Ecology of Animals
Email: krukoff@mail.ru
俄罗斯联邦, Novosibirsk