Protective Effect of Insulin on Rat Cortical Neurons in Oxidative Stress and Its Dependence on Modulation of Protein Kinase B (Akt) Activity
- Авторы: Zakharova I.1, Sokolova T.1, Zorina I.1, Bayunova L.1, Rychkova M.1, Avrova N.1
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Учреждения:
- Sechenov Institute of Evolutionary Physiology and Biochemistry
- Выпуск: Том 54, № 3 (2018)
- Страницы: 192-204
- Раздел: Comparative and Ontogenic Biochemistry
- URL: https://journals.rcsi.science/0022-0930/article/view/159481
- DOI: https://doi.org/10.1134/S0022093018030043
- ID: 159481
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Аннотация
Clinical trials of insulin and experiments on its intranasal administration to animals suggest that this hormone can be efficient in treating human neurodegenerative and some other diseases associated with brain injury. However, the mechanism of the neuroprotective effect of intranasal insulin is far from being understood. The aim of the present work was to study the protective and antioxidative effects of insulin at various concentrations on rat brain cortical neurons under oxidative stress conditions and to estimate the contribution of protein kinase B (Akt) activity modulation to insulin-induced enhancement of neuronal viability in the rat brain cortex. The protective effect of insulin was shown to be dose-dependent within the nanomolar range (1 nM < 10 nM < 100 nM and/or 1 μM). A study of the antioxidative effect of insulin revealed the efficacy of such a low concentration as 1 nM. Immunoblot analysis showed that insulin at concentrations of 100 nM and 1 μM activates Akt both in neurons and control cells at different times after their exposure to a pro-oxidant agent. LY294002, a specific PI3K/Akt signaling pathway inhibitor, was shown to significantly reduce the protective and antioxidative effects of insulin. Insulin-induced upregulation both of Akt activity and antiapoptotic protein Bcl-2 appears to play an important role in the neuroprotective effect of insulin.
Об авторах
I. Zakharova
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: avrova@iephb.ru
Россия, St. Petersburg
T. Sokolova
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: avrova@iephb.ru
Россия, St. Petersburg
I. Zorina
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: avrova@iephb.ru
Россия, St. Petersburg
L. Bayunova
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: avrova@iephb.ru
Россия, St. Petersburg
M. Rychkova
Sechenov Institute of Evolutionary Physiology and Biochemistry
Email: avrova@iephb.ru
Россия, St. Petersburg
N. Avrova
Sechenov Institute of Evolutionary Physiology and Biochemistry
Автор, ответственный за переписку.
Email: avrova@iephb.ru
Россия, St. Petersburg