Antimicrobial effects of α-defensins from leukocytes of the hamadryas baboon Papio hamadryas
- Авторы: Tsvetkova E.1, Leonova L.1, Aleshina G.2, Shamova O.1,2, Romanovskaya E.1, Mavropulo-Stolyarenko G.1, Kokryakov V.1,2
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Учреждения:
- Saint-Petersburg State University
- Institute of Experimental Medicine RAMS
- Выпуск: Том 52, № 2 (2016)
- Страницы: 133-140
- Раздел: Comparative and Ontogenic Biochemistry
- URL: https://journals.rcsi.science/0022-0930/article/view/159012
- DOI: https://doi.org/10.1134/S0022093016020046
- ID: 159012
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Аннотация
To understand the emergence and evolutionary selection of the efficient mechanisms of innate immunity it is necessary to accumulate knowledge about the structural and functional properties of antimicrobial peptides in different animal species. The cationic antimicrobial peptides, α-defensins, were isolated from leukocytic extracts of the lower narrow-nosed monkey, hamadryas baboon Papio hamadryas, using ultrafiltration, preparative electrophoresis and reverse-phase highperformance liquid chromatography. Analysis of the antimicrobial properties of α-defensins showed that they display a wide spectrum of antimicrobial activity, comparable with that of human α-defensin HNP1, and exert bactericidal and fungicidal effects at micromolar concentrations. A study of the influence of different medium conditions on antimicrobial activity of α-defensins revealed that a higher ionic strength or the presence of blood serum leads to a marked decrease in antimicrobial activity of α-defensins, while pH has no appreciable effect on it. We found that hamadryas baboon α-defensins are able to increase the permeability of the outer and inner membranes of E. coli, suggests that the bacterial membrane is one of the major targets of the antimicrobial effects of these peptides. The revealed differences in antimicrobial activity of α-defensins may result from their structural heterogeneity, which reflects different pathways of evolution of α-defensins in primates and underlies the selectivity of their antimicrobial effect.
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Об авторах
E. Tsvetkova
Saint-Petersburg State University
Автор, ответственный за переписку.
Email: e.v.tsvetkova@spbu.ru
Россия, Saint-Petersburg
L. Leonova
Saint-Petersburg State University
Email: e.v.tsvetkova@spbu.ru
Россия, Saint-Petersburg
G. Aleshina
Institute of Experimental Medicine RAMS
Email: e.v.tsvetkova@spbu.ru
Россия, Saint-Petersburg
O. Shamova
Saint-Petersburg State University; Institute of Experimental Medicine RAMS
Email: e.v.tsvetkova@spbu.ru
Россия, Saint-Petersburg; Saint-Petersburg
E. Romanovskaya
Saint-Petersburg State University
Email: e.v.tsvetkova@spbu.ru
Россия, Saint-Petersburg
G. Mavropulo-Stolyarenko
Saint-Petersburg State University
Email: e.v.tsvetkova@spbu.ru
Россия, Saint-Petersburg
V. Kokryakov
Saint-Petersburg State University; Institute of Experimental Medicine RAMS
Email: e.v.tsvetkova@spbu.ru
Россия, Saint-Petersburg; Saint-Petersburg