Genetic alterations of oral cancer associated with progression in young adults

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Abstract

Oral squamous cell carcinoma (OSCC) in young adults is an aggressive and rapidly progressive disease that is not correlated with traditional risk factors such as smoking, alcohol consumption, or human papillomavirus (HPV) infection. The aim of the study was to identify genetic alterations associated with early-onset OSCC progression. The study included 25 young patients with OSCC living in the Russian Federation. Whole-exome sequencing of primary tumor and peripheral blood samples was performed using the SureSelect XT v. 8.0 DNA library preparation kit on the Genolab M platform. Analysis of single nucleotide variants (SNVs) and insertions and deletions (indels) was performed using the GATK pipeline; filtering of genetic variants in the tumor relative to those in the peripheral blood was performed using the Mutect2 pipeline; mutation annotation was performed using ANNOVAR. Statistical data processing was performed using the IBM SPSS Statistics 20 computer program package. To test mutations that showed a statistically significant result in relation to relapse-free, metastasis-free, and overall survival, data from the Cancer Genome Atlas (N = 127) were used. There was revealed the prognostic significance of somatic mutations in TAF1L, CUL7, PRPS1L1, and CDKN2A genes for relapse-free survival (p = 0.003, p = 0.003, p = 0.003, and p = 0.004 respectively), PLEC, and TEP1 for metastasis-free survival (p < 0.001 and p = 0.007 respectively), and ADGRL3, PREX1, and CDKN2A for overall survival (p = 0.023, p = 0.023, and p < 0.001 respectively). All mutations in the CDKN2A gene were frameshift or results in a premature termination codon, one patient was found to have a pathogenic variant rs121913387. For the CUL7 gene, the variant rs369167170 was recurrent in patients with relapses, and for the PLEC gene, the variant in the region 7:18027430 C>A was recurrent in patients with metastases. Analysis of The Cancer Genome Atlas data confirmed the association of only CDKN2A mutations with OSCC progression at the age of 45 years (N = 18, p = 0.049), but not in older patients (N = 109). This work revealed genetic alterations associated with the progression of early-onset OSCC, which require confirmation of their significance in a larger sample of patients and an assessment of their functional significance.

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About the authors

E. S. Kolegova

Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences

Author for correspondence.
Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Tomsk, 634009

A. A. Korobeynikova

Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Tomsk, 634009

R. S. Vorobev

Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Tomsk, 634009

I. K. Fedorova

Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Tomsk, 634009

D. E. Kulbakin

Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Tomsk, 634009

G. A. Kononova

Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Tomsk, 634009

L. D. Zhuikova

Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Tomsk, 634009

A. P. Polyakov

P. Herzen Moscow Oncology Research Institute – a branch of the National Medical Radiology Research Center, Ministry of Health of Russia

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Moscow, 125284

A. V. Mordovsky

P. Herzen Moscow Oncology Research Institute – a branch of the National Medical Radiology Research Center, Ministry of Health of Russia

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Moscow, 125284

I. V. Novikova

P. Herzen Moscow Oncology Research Institute – a branch of the National Medical Radiology Research Center, Ministry of Health of Russia

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Moscow, 125284

A. D. Kaprin

P. Herzen Moscow Oncology Research Institute – a branch of the National Medical Radiology Research Center, Ministry of Health of Russia

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Moscow, 125284

P. A. Shatalov

P. Herzen Moscow Oncology Research Institute – a branch of the National Medical Radiology Research Center, Ministry of Health of Russia

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Moscow, 125284

P. V. Shegai

P. Herzen Moscow Oncology Research Institute – a branch of the National Medical Radiology Research Center, Ministry of Health of Russia

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Moscow, 125284

L. P. Yakovleva

The Loginov Moscow Clinical Scientific Center, Moscow Healthcare Department

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Moscow, 111123

A. S. Vyalov

The Loginov Moscow Clinical Scientific Center, Moscow Healthcare Department

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Moscow, 111123

V. T. Tsiklauri

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Moscow, 115522

O. A. Saprina

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Moscow, 115522

M. A. Kropotov

N.N. Blokhin National Medical Research Center of Oncology, Ministry of Health of the Russian Federation

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Moscow, 115522

N. G. Suchkova

The Burdenko Main Military Clinical Hospital of the Ministry of Defense of the Russian Federation

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Moscow, 105094

N. S. Sukortseva

I.M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Moscow, 119048

S. I. Samoilova

I.M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Moscow, 119048

I. V. Reshetov

I.M. Sechenov First Moscow State Medical University, Ministry of Health of the Russian Federation

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Moscow, 119048

E. L. Choinzonov

Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Tomsk, 634009

E. V. Denisov

Cancer Research Institute, Tomsk National Research Medical Center, Russian Academy of Sciences

Email: elenakolegova@oncology.tomsk.ru

The Consortium "Etiology and pathogenesis of oral cancer in young adults"

Russian Federation, Tomsk, 634009

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Supplementary files

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2. Fig. 1. Study design.

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3. Fig. 2. One-year relapse-free survival rates of young patients with PCPR depending on mutations in the TAF1L, CUL7, PRPS1L1 and CDKN2A genes.

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4. Fig. 3. One-year metastasis-free survival rates of young patients with PKC depending on mutations in the PLEC and TEP1 genes.

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5. Fig. 4. One-year overall survival rates of young patients with PCPR depending on mutations in the ADGRL3, PREX1 and CDKN2A genes.

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6. Appendix
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