Analysis of Genome Variability of Escherichia coli when Exposed to Ionizing Radiation
- Authors: Gallyamova M.Y.1, Vagin K.N.1,2, Vasilevsky N.M.1, Hammadov N.I.1
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Affiliations:
- Federal Center for Toxicological, Radiation and Biological Safety
- Kazan (Volga Region) Federal University
- Issue: Vol 60, No 1 (2024)
- Pages: 62-68
- Section: ОБЩАЯ ГЕНЕТИКА
- URL: https://journals.rcsi.science/0016-6758/article/view/255584
- DOI: https://doi.org/10.31857/S0016675824010041
- ID: 255584
Cite item
Abstract
The study of changes in the genetic apparatus of biological systems due to the influence of physical factors contributes to understanding the mechanisms of adaptation. The article is devoted to the analysis of the genome of the modified Escherichia coli PL-6 variant obtained as a result of repeated and gradually increasing exposure to 60Co ɤ-rays at the Researcher facility. The integrity of the genetic material of the studied bacterial cells was checked by electrophoresis in 1.7% agarose gel. For the analysis of genome modifications, primers were designed to amplify several loci characterized by homology in a variety of E. coli strains. Based on the indicators of the number and size of amplified products using each of the presented primer combinations in E. coli before and after gamma irradiation, a significant change in the genome was established.
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About the authors
M. Yu. Gallyamova
Federal Center for Toxicological, Radiation and Biological Safety
Author for correspondence.
Email: kostya9938@yandex.ru
Russian Federation, Kazan
K. N. Vagin
Federal Center for Toxicological, Radiation and Biological Safety; Kazan (Volga Region) Federal University
Email: kostya9938@yandex.ru
Russian Federation, Kazan; Kazan
N. M. Vasilevsky
Federal Center for Toxicological, Radiation and Biological Safety
Email: kostya9938@yandex.ru
Russian Federation, Kazan
N. I. Hammadov
Federal Center for Toxicological, Radiation and Biological Safety
Email: kostya9938@yandex.ru
Russian Federation, Kazan
References
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