A Robust Test System for DNA Identification of Raccoon Dogs

A. E. Hrebianchuk; Гребенчук А. Е.; O. N. Lukashkova; Лукашкова О. Н.; S. A. Kotava; Котова С. А.; I. S. Tsybovsky; Цыбовский И. С.

doi:10.31857/S0016675823050065

A Robust Test System for DNA Identification of Raccoon Dogs

Authors: Hrebianchuk A.E.¹, Lukashkova O.N.², Kotava S.A.², Tsybovsky I.S.³
Affiliations:
1. State Forensic Examination Committee of the Republic of Belarus
2. Scientific and Practical Center of the State Forensic Examination Committee of the Republic Belarus
3. RUP “BelJurZabespjachjenne”
Issue: Vol 59, No 5 (2023)
Pages: 584-600
Section: МАТЕМАТИЧЕСКИЕ МОДЕЛИ И МЕТОДЫ
URL: https://journals.rcsi.science/0016-6758/article/view/134591
DOI: https://doi.org/10.31857/S0016675823050065
EDN: https://elibrary.ru/FNOVHT
ID: 134591

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Abstract
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Abstract

To establish a system for DNA identification of biological samples of the raccoon dog (Nyctereutes procyonoides), we carried out a study of polymorphism of 39 microsatellite loci and three sex loci specific to various species of the Canidae family. This resulted in the development of the NPlex test system, which includes 14 autosomal STRs and two sex loci. The test system has been validated for use in forensic identification of racoon dogs based on biological traces (such as traces of blood and secretions, fragments of muscle tissue or bones, dermis and hair, etc.) found at crime scenes, as well as for establishing biological relationship of animals of this species. Validation efforts were performed in accordance with the protocol of the Scientific Working Group on DNA Analysis Methods.

Keywords

microsatellites, identification, Canidae family, forensics, illegal hunting.

References

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