Variability of 27 Autosomal STR Loci for the Population of the Republic of Belarus Based on the Mass Parallel Sequencing Data

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Abstract

Variability of 27 autosomal STR loci of the ForenSeq DNA Signature Prep Kit (Illumina) commercial panel was studied using the technology of mass parallel sequencing (MPS) in 733 unrelated individuals representing the population of the Republic of Belarus as well as a population base of MPS allele frequencies for expert probabilistic calculations in human identification and paternity establishment was evaluated. The agreement between genotypes obtained by MPS and capillary electrophoresis (CE) was 99.96%. The number of MPS alleles increased more than two times for eight loci (D12S391, D21S11, D2S1338, vWA, D3S1358, D8S1179, D13S317, D9S1122). Thirteen alleles detected were not included in the STRSeq BioProject catalog of the international online database STRbase 2.0. The random match probability of 27-locus MPS profiles decreased from 1.43 × 10–31 to 2.89 × 10–35, and the combined paternity index increased from 2.08 × 1010 to 3.25 × 1012 compared to CE data.

About the authors

S. A. Kotova

Scientific and Practical Center of the State Forensic Examination Committee
of the Republic of Belarus

Author for correspondence.
Email: svetlkotova@mail.ru
Republic of Belarus, 220114, Minsk

N. S. Parfionava

Scientific and Practical Center of the State Forensic Examination Committee
of the Republic of Belarus

Email: svetlkotova@mail.ru
Republic of Belarus, 220114, Minsk

T. V. Zabauskaya

Scientific and Practical Center of the State Forensic Examination Committee
of the Republic of Belarus

Email: svetlkotova@mail.ru
Republic of Belarus, 220114, Minsk

V. I. Rybakova

Scientific and Practical Center of the State Forensic Examination Committee
of the Republic of Belarus

Email: svetlkotova@mail.ru
Republic of Belarus, 220114, Minsk

A. A. Spivak

Scientific and Practical Center of the State Forensic Examination Committee
of the Republic of Belarus

Email: svetlkotova@mail.ru
Republic of Belarus, 220114, Minsk

S. A. Paliavoi

Masaryk University

Email: svetlkotova@mail.ru
Czech Republic, 60177, Brno

A. V. Lugovnev

State Forensic Examination Committee of the Republic
of Belarus

Email: svetlkotova@mail.ru
Republic of Belarus, 220073, Minsk

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Copyright (c) 2023 С.А. Котова, А.С. Парфёнова, Т.В. Забавская, В.И. Рыбакова, Е.А. Спивак, С.А. Полевой, А.В. Луговнёв

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