Analysis of  ADCY8  и  RYR3  Genes Polymorphism for Identification Wild and Domestic Animal of Species  Canis lupus

V. N. Kipen; Кипень В. Н.; M. M. Patrin; Патрин М. М.; E. V. Snytkov; Снытков Е. В.; A. N. Viarchuk; Верчук А. Н.; A. N. Semak; Семак А. Н.

doi:10.31857/S0016675823030062

Analysis of ADCY8 и RYR3 Genes Polymorphism for Identification Wild and Domestic Animal of Species Canis lupus

Authors: Kipen V.N.¹, Patrin M.M.², Snytkov E.V.¹^,3, Viarchuk A.N.¹^,4, Semak A.N.⁵
Affiliations:
1. Institute of Genetics and Cytology of the National Academy of Sciences of Belarus
2. Maxim Medical LLC
3. International Sakharov Environmental Institute of Belarusian State University
4. Scientific and Practical Center of the State Forensic Examination Committee of the Republic of Belarus
5. WellVet LLC
Issue: Vol 59, No 3 (2023)
Pages: 336-344
Section: ГЕНЕТИКА ЖИВОТНЫХ
URL: https://journals.rcsi.science/0016-6758/article/view/134570
DOI: https://doi.org/10.31857/S0016675823030062
EDN: https://elibrary.ru/IOHKSC
ID: 134570

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Abstract
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Abstract

As a result of the study, we confirmed the high differentiating potential of polymorphic variants g.27748425T>C (ADCY8 gene) and g.1414373T>C (RYR3 gene). A test model of two polymorphisms for the differentiation of a wolf and a domestic dog is proposed, which is distinguished by high values of accuracy (96.2%), specificity (96.3%) and sensitivity (98.9%). Using KASP, a quick and simple approach to differentiation based on the proposed test model has been developed, which is designed to reduce the time and cost of molecular genetic analysis, as well as reduce the risk of cross-contamination, because the process is one-stage (restriction and electrophoresis stages are excluded).

Keywords

Canis lupus, Canis lupus familiaris, SNP, differentiation, kompetitive allele-specific PCR (KASP), RFLP-PCR, high-resolution amplicon melting (HRM), in silico genotyping.

References

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