Характеристика нового гена монодегидроаскорбатредуктазы у кукурузы (Zea mays L.) и его роль в ответе на стрессы

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Abstract

Растения кукурузы Zea mays L. чувствительны ко многим стрессовым факторам, вызывающим избыточное образование активных форм кислорода, механизм нивелирования воздействия которых на организм включает фермент монодегидроаскорбатредуктазу (MDHAR; EC 1.6.5.4), катализирующий восстановление монодегидроаскорбиновой кислоты до аскорбата. В геноме кукурузы известно четыре гена ZmMDHAR1–ZmMDHAR4, различающихся внутриклеточной локализацией кодируемых белков MDHAR. В данной работе был идентифицирован новый ген семейства – ZmMDHAR5 (Zm00001d017786; LOC100193942), кодирующий близкий структурный гомолог пероксисомального ZmMDHAR3. Сделано предположение, что ZmMDHAR5 может иметь функции, близкие к хлоропласт-митохондриальным MDHAR. Дифференциальная экспрессия генов ZmMDHAR1–ZmMDHAR5 в проростках кукурузы в ответ на различные стрессовые факторы позволила также предположить важное участие ZmMDHAR4 и ZmMDHAR5 в ответе на экзогенную абсцизовую кислоту, низкие температуры и обезвоживание; ZmMDHAR1 и ZmMDHAR3 – на солевой стресс; ZmMDHAR2 – на избыток соли и дефицит воды.

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М. А. Филюшин

Федеральный исследовательский центр “Фундаментальные основы биотехнологии” Российской академии наук

Author for correspondence.
Email: michel7753@mail.ru

Институт биоинженерии

Russian Federation, Москва

Д. Х. Архестова

Федеральный исследовательский центр “Фундаментальные основы биотехнологии” Российской академии наук; Федеральный научный центр “Кабардино-Балкарский научный центр Российской академии наук”

Email: michel7753@mail.ru

Институт биоинженерии, Институт сельского хозяйства

Russian Federation, Москва; Нальчик

Е. З. Кочиева

Федеральный исследовательский центр “Фундаментальные основы биотехнологии” Российской академии наук

Email: michel7753@mail.ru

Институт биоинженерии

Russian Federation, Москва

А. В. Щенникова

Федеральный исследовательский центр “Фундаментальные основы биотехнологии” Российской академии наук

Email: michel7753@mail.ru

Институт биоинженерии

Russian Federation, Москва

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Supplementary files

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2. Fig. 1. Variants (1-4) of the ZmMDHAR3–ZmMDHAR5 fragment on chromosome 5 in the genome of 35 maize samples, including the exon-intron structure of the ZmMDHAR3 genes (exons are indicated by blue blocks) and ZmMDHAR5 (exons are indicated by pink blocks); the red line indicates the intergenic spacer.

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3. Fig. 2. Alignment of sequences of proteins of the ZmMDHAR family. The Pyr_redox_2 domain (pfam07992) is underlined in red. The binding sites with FAD/NAD(P)H cofactors are highlighted in black. The consensus GxGxxG/GxGxxxG is highlighted in blue.

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4. Fig. 3. a is a phylogenetic tree based on sequences of MDHARs proteins of rice O. sativa (OsMDHAR1 (NP_001404045.1), OsMDHAR2 (NP_001403773.1), OsMDHAR3 (XP_015611766.1), OsMDHAR4 (NP_001390619.1), OsMDHAR5 (XP_015649952.1)), Asparagus As. officinalis (AoMDHAR1 (XP_020260315.1), AoMDHAR4 (XP_020251572.1), AoMDHAR5 (XP_020241046.1)), garlic A. sativum (AsMDHAR1 (Asa7G02482.1), AsMDHAR4 (Asa0G05211.1), AsMDHAR5 (Asa5G00445.1)), A. thaliana (AtMDHAR1 (At3g52880, NP_190856), AtMDHAR2 (At5g03630, NP_568125.1), AtMDHAR3 (At3g09940, NP_566361.1), AtMDHAR4 (At3g27820, NP_189420.1), AtMDHAR6 (At1g63940, NP_849839.1)) and P. patens ( PpMDHAR1 (ABA47446.1), PpMDHAR2 (ABA47447.1) and PpMDHAR3 (ABA47448.1)). The dendrogram was built using the MEGA 7.0 program (Maximum Likelihood method, 1000 bootstrap replicas). b – Conservative motifs identified using MEME 5.1.1 in MDHAR proteins.

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5. Fig. 4. Differential expression of ZmMDHAR genes in response to treatment with ABA (100 microns), cold stress (+3°C), salt stress (NaCl, 250 mM) and dehydration (10% PEG-6000) for 6 and 24 hours. The raw control is accepted as 1.

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6. Supplementary information
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Note

1 Дополнительные материалы размещены в электронном виде по DOI статьи: 10.31857/S0015330324010067


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