Isolation of CP-PVY-Specific siRNA from PVY-Infected Plants of Solanum tuberosum

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Abstract

Tools for activating crop resistance to viruses are now becoming part of a comprehensive plant protection

strategy. Artificial resistance to viruses through expression of the viral envelope protein in transgenic

plants is fairly well understood. An urgent issue is the study of small RNAs involved in the protective mechanisms

of RNA interference against viruses. Understanding the role of short interfering RNA (siRNA) in the

regulation and shutdown of genes is important. The proteinase accessory component (HC-Pro), a multifunctional

suppressor protein synthesized by the potato virus Y, is able to neutralize S. tuberosum plant defenses

by trapping siRNA and removing them from the RNA interference process, thereby causing systemic infection

of the host plant. Protein liquid chromatography combined with high performance sequencing can help

recognize the large number of small RNAs resulting from viral RNA degradation and identify 21–23 bp.

siRNA from PVY-infected S. tuberosum plants. The HC-Pro/siRNA nucleoprotein complex was detected in

chromatographic fractions using antibodies against HC-Pro, Southern-blot indicated the presence of small

RNAs in the complex, and analysis of data from deep sequencing of the small RNA population determined a

specificity of 21–23 bp. siRNA to the envelope protein of the PVY virus. The research results can be applied

in the study of intracellular signaling molecules and stimulate new research on antiviral mechanisms to

develop effective strategies for plant protection against viruses.

About the authors

M. Yu. Sutula

Non-commercial joint stock company “East Kazakhstan University named after Sarsen Amanzholov”

Email: max.sutula@gmail.com
Ust-Kamenogorsk, Kazakhstan

Zh. K. Kabataeva

Non-commercial joint stock company “East Kazakhstan University named after Sarsen Amanzholov”

Email: max.sutula@gmail.com
Ust-Kamenogorsk, Kazakhstan

G. K. Komekova

Non-commercial joint stock company “East Kazakhstan University named after Sarsen Amanzholov”

Email: max.sutula@gmail.com
Ust-Kamenogorsk, Kazakhstan

T. S. Khosnutdinova

Non-commercial joint stock company “East Kazakhstan University named after Sarsen Amanzholov”

Email: max.sutula@gmail.com
Ust-Kamenogorsk, Kazakhstan

E. A. Zhakmanova

Non-commercial joint stock company “East Kazakhstan University named after Sarsen Amanzholov”

Author for correspondence.
Email: max.sutula@gmail.com
Ust-Kamenogorsk, Kazakhstan

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