Differential Expression of Sugar Uniporter Genes of the SWEET Family in the Regulation of Qualitative Fruit Traits in Tomato Species (Solanum Section Lycopersicon)

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Abstract

Tomato (Solanum lycopersicum L.) is an important crop and, due to the existence of wild related
species (Solanum section Lycopersicon), a model for studying the development of the fleshy fruit. In the
study, expression analysis of sugar uniporter genes SWEET1а, 1b, 1e, 3, 7a, 10a, 12c, 14, and 15 in tomato
species and cultivars was carried out. In cv. Heinz (S. lycopersicum), genes that are most active in roots
(SWEET1e, 3, 10a, and 12c), leaves (SWEET1a, 1e, 3, 10a, and 12c) and flowers (SWEET1a, 1b, 7a, 10a,
12s, 14, and 15) were revealed. The growth of the fruit is accompanied by an increase in the level of the
SWEET 110a and 12c transcripts; maturation of the fruit is accompanied by an increase in the level of the
SWEET 1a and 15 transcripts. Differential expression of the SWEET1a, 1b, 12c, and 15 genes in the ripe fruit
of inbred lines obtained from crossing of S. lycopersicum cv. M82 × S. pennellii was demonstrated. qRT-PCR
analysis showed that the expression of the SWEET1a and 12c genes is common for ripe fruit of the analyzed
tomato species, while the expression of the SWEET1b and 10a genes is common for S. pennellii, S. habrochaites,
and S. cheesmaniae. It was determined that the fructose : glucose ratio is equimolar in the accessions
except for cv. Black Jack and White Beauty (fructose : glucose ≥ 1.10). Correlations between the level of
SWEET gene transcripts and the ratio of hexoses was not revealed.

About the authors

M. A. Filyushin

Federal Research Center Fundamentals of Biotechnology, Russian Academy of Sciences

Email: michel7753@mail.ru
Moscow, Russia

M. A. Slugina

Federal Research Center Fundamentals of Biotechnology, Russian Academy of Sciences

Email: michel7753@mail.ru
Moscow, Russia

A. V. Shchennikova

Federal Research Center Fundamentals of Biotechnology, Russian Academy of Sciences

Email: michel7753@mail.ru
Moscow, Russia

E. Z. Kochieva

Federal Research Center Fundamentals of Biotechnology, Russian Academy of Sciences

Author for correspondence.
Email: michel7753@mail.ru
Moscow, Russia

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