Effect of Expression of the NDB2 Heterologous Gene 
of Arabidopsis thaliana on Growth and Respiratory Activity 
of Nicotiana tabacum

N. E. Korotaeva; Коротаева Н. Е.; A. M. Shigarova; Шигарова А. М.; A. I. Katyshev; Катышев А. И.; I. V. Fedoseeva; Федосеева И. В.; A. V. Fedyaeva; Федяева А. В.; D. V. Sauchyn; Савчин Д. В.; A. M. Shyshlova-Sokolovskaya; Шишлова-Соколовская А. М.; O. Yu. Urbanovich; Урбанович О. Ю.; G. B. Borovskii; Боровский Г. Б.

doi:10.31857/S0015330323600031

Effect of Expression of the NDB2 Heterologous Gene of Arabidopsis thaliana on Growth and Respiratory Activity of Nicotiana tabacum

Authors: Korotaeva N.E.¹, Shigarova A.M.¹, Katyshev A.I.¹, Fedoseeva I.V.¹, Fedyaeva A.V.¹^,2, Sauchyn D.V.³, Shyshlova-Sokolovskaya A.M.³, Urbanovich O.Y.³, Borovskii G.B.⁴
Affiliations:
1. Siberian Institute of Plant Physiology and Biochemistry, Siberian Branch, Russian Academy of Sciences
2. Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences
3. Institute of Genetics and Cytology, National Academy of Sciences of Belarus
4. Федеральное государственное бюджетное научное учреждение Сибирский институт физиологии и биохимии растений Сибирского отделения Российской академии наук
Issue: Vol 70, No 5 (2023)
Pages: 461-471
Section: ЭКСПЕРИМЕНТАЛЬНЫЕ СТАТЬИ
URL: https://journals.rcsi.science/0015-3303/article/view/139774
DOI: https://doi.org/10.31857/S0015330323600031
EDN: https://elibrary.ru/PBSFBL
ID: 139774

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Abstract
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Abstract

Novel lines of tobacco (Nicotiana tabacum L.), highly expressing the AtNDB2 gene (NDB2 from Arabidopsis thaliana (L.) Heynh.), were produced with the help of agrobacterial transformation followed by a selection. The transgenic 13s line, possessing typical exterior and the AtNDB2 expression level, was compared with the initial wild type of N. tabacum regarding the parameters of growth and respiratory activity under optimal and suboptimal temperatures. It was found that the total and alternative respiration increased and the superoxide anion generation decreased in the 13s plants under the suboptimal temperature. The growth rate was decreased in the plants highly expressing the AtNDB2 gene in comparison with the control wild type plants, especially at the temperature below the optimum. Possible causes of the found changes are discussed.

Keywords

Arabidopsis thaliana, Nicotiana tabacum, AtNDB2, ROS, agrobacterial transformation, respiratory activity, growth rate

References

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