Effect of the clathrate hydrate environment on the structure of collagen xenograft tissue with chitosan coating deposited from solution in the H2O/CO2 system at high pressure

Abstract

The effect of the clathrate hydrate environment in the water/carbon dioxide system on the structure of the chitosan-coated collagen xenograft tissue was demonstrated. The collagen xenograft tissue treated in this environment is a porous material with a pore size of 50–500 nm having also micrometer size (1–3 μm) pores. The high porosity results in averaging of the structural organization features in xenograft tissues with different orientation of collagen fibrils. Treatment of xenograft tissues in the H₂O/CO₂ environment in the presence of clathrate hydrates allows the manufacture of a nanoporous biodegradable material suitable for biomedical applications.