The Role of Urokinase, Tumor Necrosis Factor, and Matrix Metalloproteinase-9 in Monocyte Activation
- 作者: Menshikov M.1, Stafeev I.1, Zubkova E.1, Beloglazova I.1, Ratner E.1, Dergilev K.1, Parfyonova E.1
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隶属关系:
- National Research Center for Cardiology, Health Ministry of Russian Federation
- 期: 卷 167, 编号 4 (2019)
- 页面: 492-495
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241817
- DOI: https://doi.org/10.1007/s10517-019-04557-2
- ID: 241817
如何引用文章
详细
TNFα mediates the expression of MMP-9 in THP-1 monocytes induced by urokinase (uPA). Upregulation of MMP-9 caused by uPA and TNFα is suppressed by etanercept, a TNFα inhibitor. In addition, uPA stimulates TNFα mRNA expression. Both uPA and TNFα induce ROS generation in monocytes, while MMP-9 secretion induced by uPA and TNFα is inhibited by antioxidants. Inhibitors of NFκB, ligands of PPARα and PPARγ receptors, and SIRT1 activators negatively affect MMP-9 secretion induced by uPA. MMP-9 secretion during monocyte differentiation into macrophages is downregulated by etanercept and antioxidants. These factors as well as MMP inhibitor GM6001 reduce the number of macrophages attached to substrate during cell differentiation, indicating the role of urokinase, TNFα, and ROS in MMP expression in monocytes and MMP involvement in macrophage maturation.
作者简介
M. Menshikov
National Research Center for Cardiology, Health Ministry of Russian Federation
编辑信件的主要联系方式.
Email: myumensh@mail.ru
俄罗斯联邦, Moscow
I. Stafeev
National Research Center for Cardiology, Health Ministry of Russian Federation
Email: myumensh@mail.ru
俄罗斯联邦, Moscow
E. Zubkova
National Research Center for Cardiology, Health Ministry of Russian Federation
Email: myumensh@mail.ru
俄罗斯联邦, Moscow
I. Beloglazova
National Research Center for Cardiology, Health Ministry of Russian Federation
Email: myumensh@mail.ru
俄罗斯联邦, Moscow
E. Ratner
National Research Center for Cardiology, Health Ministry of Russian Federation
Email: myumensh@mail.ru
俄罗斯联邦, Moscow
K. Dergilev
National Research Center for Cardiology, Health Ministry of Russian Federation
Email: myumensh@mail.ru
俄罗斯联邦, Moscow
E. Parfyonova
National Research Center for Cardiology, Health Ministry of Russian Federation
Email: myumensh@mail.ru
俄罗斯联邦, Moscow