The Role of Urokinase, Tumor Necrosis Factor, and Matrix Metalloproteinase-9 in Monocyte Activation
- Autores: Menshikov M.1, Stafeev I.1, Zubkova E.1, Beloglazova I.1, Ratner E.1, Dergilev K.1, Parfyonova E.1
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Afiliações:
- National Research Center for Cardiology, Health Ministry of Russian Federation
- Edição: Volume 167, Nº 4 (2019)
- Páginas: 492-495
- Seção: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241817
- DOI: https://doi.org/10.1007/s10517-019-04557-2
- ID: 241817
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Resumo
TNFα mediates the expression of MMP-9 in THP-1 monocytes induced by urokinase (uPA). Upregulation of MMP-9 caused by uPA and TNFα is suppressed by etanercept, a TNFα inhibitor. In addition, uPA stimulates TNFα mRNA expression. Both uPA and TNFα induce ROS generation in monocytes, while MMP-9 secretion induced by uPA and TNFα is inhibited by antioxidants. Inhibitors of NFκB, ligands of PPARα and PPARγ receptors, and SIRT1 activators negatively affect MMP-9 secretion induced by uPA. MMP-9 secretion during monocyte differentiation into macrophages is downregulated by etanercept and antioxidants. These factors as well as MMP inhibitor GM6001 reduce the number of macrophages attached to substrate during cell differentiation, indicating the role of urokinase, TNFα, and ROS in MMP expression in monocytes and MMP involvement in macrophage maturation.
Sobre autores
M. Menshikov
National Research Center for Cardiology, Health Ministry of Russian Federation
Autor responsável pela correspondência
Email: myumensh@mail.ru
Rússia, Moscow
I. Stafeev
National Research Center for Cardiology, Health Ministry of Russian Federation
Email: myumensh@mail.ru
Rússia, Moscow
E. Zubkova
National Research Center for Cardiology, Health Ministry of Russian Federation
Email: myumensh@mail.ru
Rússia, Moscow
I. Beloglazova
National Research Center for Cardiology, Health Ministry of Russian Federation
Email: myumensh@mail.ru
Rússia, Moscow
E. Ratner
National Research Center for Cardiology, Health Ministry of Russian Federation
Email: myumensh@mail.ru
Rússia, Moscow
K. Dergilev
National Research Center for Cardiology, Health Ministry of Russian Federation
Email: myumensh@mail.ru
Rússia, Moscow
E. Parfyonova
National Research Center for Cardiology, Health Ministry of Russian Federation
Email: myumensh@mail.ru
Rússia, Moscow