Effect of Heterologous Expression of Chemotaxis Proteins from Genus Thermotoga on the Growth Kinetics of Escherichia coli Cells
- 作者: Grishin D.1, Samoilenko V.2, Gladilina Y.1, Zhdanov D.1, Pokrovskaya M.1, Aleksandrova S.1, Pokrovsky V.1, Sokolov N.1
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隶属关系:
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences
- 期: 卷 167, 编号 3 (2019)
- 页面: 375-379
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241712
- DOI: https://doi.org/10.1007/s10517-019-04530-z
- ID: 241712
如何引用文章
详细
In the process of optimization of heterologous expression of thermostable chemotaxis proteins CheW and CheY as industrially useful polypeptides, their direct influence on the cell growth kinetics and morphology of Escherichia coli was observed. CheW and CheY of bacteria of the genus Thermotoga, being expressed in recombinant form in E. coli cells, are involved in the corresponding signal pathways of the mesophilic microorganisms. The effects of such involvement in the metabolism of “host” cells are extremely diverse: from rapid aging of the culture to elongation of the stationary growth phase. We also discuss the mechanisms of the influence of the heterologous chemotaxis proteins on cells, their positive and negative effects, as well as potential applications in industry and biomedicine.
作者简介
D. Grishin
V. N. Orekhovich Research Institute of Biomedical Chemistry
编辑信件的主要联系方式.
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Moscow
V. Samoilenko
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Pushchino, Moscow Region
Yu. Gladilina
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Moscow
D. Zhdanov
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Moscow
M. Pokrovskaya
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Moscow
S. Aleksandrova
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Moscow
V. Pokrovsky
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Moscow
N. Sokolov
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
俄罗斯联邦, Moscow