Effect of Heterologous Expression of Chemotaxis Proteins from Genus Thermotoga on the Growth Kinetics of Escherichia coli Cells
- Авторы: Grishin D.1, Samoilenko V.2, Gladilina Y.1, Zhdanov D.1, Pokrovskaya M.1, Aleksandrova S.1, Pokrovsky V.1, Sokolov N.1
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Учреждения:
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences
- Выпуск: Том 167, № 3 (2019)
- Страницы: 375-379
- Раздел: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241712
- DOI: https://doi.org/10.1007/s10517-019-04530-z
- ID: 241712
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Аннотация
In the process of optimization of heterologous expression of thermostable chemotaxis proteins CheW and CheY as industrially useful polypeptides, their direct influence on the cell growth kinetics and morphology of Escherichia coli was observed. CheW and CheY of bacteria of the genus Thermotoga, being expressed in recombinant form in E. coli cells, are involved in the corresponding signal pathways of the mesophilic microorganisms. The effects of such involvement in the metabolism of “host” cells are extremely diverse: from rapid aging of the culture to elongation of the stationary growth phase. We also discuss the mechanisms of the influence of the heterologous chemotaxis proteins on cells, their positive and negative effects, as well as potential applications in industry and biomedicine.
Об авторах
D. Grishin
V. N. Orekhovich Research Institute of Biomedical Chemistry
Автор, ответственный за переписку.
Email: molbiol_ibm@inbox.ru
Россия, Moscow
V. Samoilenko
G. K. Skryabin Institute of Biochemistry and Physiology of Microorganisms, Russian Academy of Sciences
Email: molbiol_ibm@inbox.ru
Россия, Pushchino, Moscow Region
Yu. Gladilina
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Россия, Moscow
D. Zhdanov
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Россия, Moscow
M. Pokrovskaya
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Россия, Moscow
S. Aleksandrova
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Россия, Moscow
V. Pokrovsky
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Россия, Moscow
N. Sokolov
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: molbiol_ibm@inbox.ru
Россия, Moscow