A Cell Model of Human Small Intestinal Wall Based on Genetically Modified Caco-2 Cells
- 作者: Rusanov A.1, Luzgina E.1, Vakhrushev I.2, Nakhod K.2, Luzgina N.1,2
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隶属关系:
- Perspektiva Company
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- 期: 卷 166, 编号 1 (2018)
- 页面: 174-177
- 栏目: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/240891
- DOI: https://doi.org/10.1007/s10517-018-4308-2
- ID: 240891
如何引用文章
详细
We propose a cell model of the human small intestinal wall based on genetically modified Caco-2 cells that allows visualization and quantitative assessment of activation of NF-κB factor and related intracellular pathway by using fluorescence microscopy. A dose-dependent increase in fluorescence intensity of the obtained cells in response to TNFα exposure in concentrations of 1-100 ng/ml was demonstrated. It was found that this parameter correlates with a decrease in the transepithelial resistance of the cell monolayer in response to TNFα and can be used to assess the toxic effects of substances on epithelial cells of the human small intestine.
作者简介
A. Rusanov
Perspektiva Company
编辑信件的主要联系方式.
Email: alexander.l.rusanov@gmail.com
俄罗斯联邦, Novosibirsk
E. Luzgina
Perspektiva Company
Email: alexander.l.rusanov@gmail.com
俄罗斯联邦, Novosibirsk
I. Vakhrushev
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: alexander.l.rusanov@gmail.com
俄罗斯联邦, Moscow
K. Nakhod
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: alexander.l.rusanov@gmail.com
俄罗斯联邦, Moscow
N. Luzgina
Perspektiva Company; V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: alexander.l.rusanov@gmail.com
俄罗斯联邦, Novosibirsk; Moscow