A Cell Model of Human Small Intestinal Wall Based on Genetically Modified Caco-2 Cells
- Authors: Rusanov A.L.1, Luzgina E.D.1, Vakhrushev I.V.2, Nakhod K.V.2, Luzgina N.G.1,2
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Affiliations:
- Perspektiva Company
- V. N. Orekhovich Research Institute of Biomedical Chemistry
- Issue: Vol 166, No 1 (2018)
- Pages: 174-177
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/240891
- DOI: https://doi.org/10.1007/s10517-018-4308-2
- ID: 240891
Cite item
Abstract
We propose a cell model of the human small intestinal wall based on genetically modified Caco-2 cells that allows visualization and quantitative assessment of activation of NF-κB factor and related intracellular pathway by using fluorescence microscopy. A dose-dependent increase in fluorescence intensity of the obtained cells in response to TNFα exposure in concentrations of 1-100 ng/ml was demonstrated. It was found that this parameter correlates with a decrease in the transepithelial resistance of the cell monolayer in response to TNFα and can be used to assess the toxic effects of substances on epithelial cells of the human small intestine.
About the authors
A. L. Rusanov
Perspektiva Company
Author for correspondence.
Email: alexander.l.rusanov@gmail.com
Russian Federation, Novosibirsk
E. D. Luzgina
Perspektiva Company
Email: alexander.l.rusanov@gmail.com
Russian Federation, Novosibirsk
I. V. Vakhrushev
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: alexander.l.rusanov@gmail.com
Russian Federation, Moscow
K. V. Nakhod
V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: alexander.l.rusanov@gmail.com
Russian Federation, Moscow
N. G. Luzgina
Perspektiva Company; V. N. Orekhovich Research Institute of Biomedical Chemistry
Email: alexander.l.rusanov@gmail.com
Russian Federation, Novosibirsk; Moscow