Evaluation of Regenerative Potential of Cryopreserved Adenosine-Modified Monocytes on the Model of Burn Wounds

K. V. Nevskaya; V. V. Ivanov; E. S. Mainagasheva; S. S. Gutor; S. P. Lezhava; A. E. Sazonov

doi:10.1007/s10517-018-4209-4

Evaluation of Regenerative Potential of Cryopreserved Adenosine-Modified Monocytes on the Model of Burn Wounds

作者: Nevskaya K.¹, Ivanov V.¹, Mainagasheva E.¹, Gutor S.¹, Lezhava S.¹, Sazonov A.¹^,2
隶属关系:
1. Siberian State Medical University, Ministry of Health of the Russian Federation
2. D. I. Mendeleev University of Chemical Technology
期: 卷 165, 编号 4 (2018)
页面: 526-529
栏目: Article
URL: https://journals.rcsi.science/0007-4888/article/view/240486
DOI: https://doi.org/10.1007/s10517-018-4209-4
ID: 240486

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We studied viability of adenosine-modified monocytes after cryopreservation and their regenerative potential. The optimal method of cell cryopreservation was the use of serum-free medium followed by storage in liquid nitrogen. Injections of adenosine-modified monocytes after 12-week cryopreservation around grade IIIB burn wound accelerated healing of the skin defect and significantly improved the histological pattern of the damage area at delayed terms.