Optical and Electron Microscopic Study of the Morphology and Ultrastructure of Biofilms Formed by Streptococcus pyogenes
- 作者: Danilova T.1, Smirnova T.1, Danilina G.2, Adzhieva A.1, Andreevskaya S.1, Shevlyagina N.1, Zhukhovitsky V.2
-
隶属关系:
- Laboratory of Indication and Ultrastructural Analysis of Microorganisms, N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of Russian Federation
- I. M. Sechenov First Moscow Medical University, Ministry of Health of the Russian Federation
- 期: 卷 165, 编号 1 (2018)
- 页面: 110-114
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240032
- DOI: https://doi.org/10.1007/s10517-018-4110-1
- ID: 240032
如何引用文章
详细
Our study confirmed the capacity of S. pyogenes strains to form biofilms on abiotic surfaces. Chains of streptococci surrounded by bluish film were seen under a microscope after alcian blue staining of the preparations grown on slides. On ultrathin sections in transmission electron microscope, the extracellular matrix (indicator of biofilm maturity) became visible after staining with alcian blue. Microscopy of the sections shows structures characteristic of a biofilm in spaces between the cells. Scanning electron microscopy also demonstrates the presence of a biomembrane. Importantly that type 1M strain forming in fact no membranes when cultured on plastic plates (Costar) formed biofilms on the glass. It seems that the conditions for the biofilm formation on the plastic and on the glass differ, due to which the exopolymeric matrices formed on different surfaces vary by biochemical composition.
作者简介
T. Danilova
Laboratory of Indication and Ultrastructural Analysis of Microorganisms, N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of Russian Federation
编辑信件的主要联系方式.
Email: dansen@inbox.ru
俄罗斯联邦, Moscow
T. Smirnova
Laboratory of Indication and Ultrastructural Analysis of Microorganisms, N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of Russian Federation
Email: dansen@inbox.ru
俄罗斯联邦, Moscow
G. Danilina
I. M. Sechenov First Moscow Medical University, Ministry of Health of the Russian Federation
Email: dansen@inbox.ru
俄罗斯联邦, Moscow
A. Adzhieva
Laboratory of Indication and Ultrastructural Analysis of Microorganisms, N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of Russian Federation
Email: dansen@inbox.ru
俄罗斯联邦, Moscow
S. Andreevskaya
Laboratory of Indication and Ultrastructural Analysis of Microorganisms, N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of Russian Federation
Email: dansen@inbox.ru
俄罗斯联邦, Moscow
N. Shevlyagina
Laboratory of Indication and Ultrastructural Analysis of Microorganisms, N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of Russian Federation
Email: dansen@inbox.ru
俄罗斯联邦, Moscow
V. Zhukhovitsky
I. M. Sechenov First Moscow Medical University, Ministry of Health of the Russian Federation
Email: dansen@inbox.ru
俄罗斯联邦, Moscow