Optical and Electron Microscopic Study of the Morphology and Ultrastructure of Biofilms Formed by Streptococcus pyogenes
- Authors: Danilova T.A.1, Smirnova T.A.1, Danilina G.A.2, Adzhieva A.A.1, Andreevskaya S.G.1, Shevlyagina N.V.1, Zhukhovitsky V.G.2
-
Affiliations:
- Laboratory of Indication and Ultrastructural Analysis of Microorganisms, N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of Russian Federation
- I. M. Sechenov First Moscow Medical University, Ministry of Health of the Russian Federation
- Issue: Vol 165, No 1 (2018)
- Pages: 110-114
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/240032
- DOI: https://doi.org/10.1007/s10517-018-4110-1
- ID: 240032
Cite item
Abstract
Our study confirmed the capacity of S. pyogenes strains to form biofilms on abiotic surfaces. Chains of streptococci surrounded by bluish film were seen under a microscope after alcian blue staining of the preparations grown on slides. On ultrathin sections in transmission electron microscope, the extracellular matrix (indicator of biofilm maturity) became visible after staining with alcian blue. Microscopy of the sections shows structures characteristic of a biofilm in spaces between the cells. Scanning electron microscopy also demonstrates the presence of a biomembrane. Importantly that type 1M strain forming in fact no membranes when cultured on plastic plates (Costar) formed biofilms on the glass. It seems that the conditions for the biofilm formation on the plastic and on the glass differ, due to which the exopolymeric matrices formed on different surfaces vary by biochemical composition.
About the authors
T. A. Danilova
Laboratory of Indication and Ultrastructural Analysis of Microorganisms, N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of Russian Federation
Author for correspondence.
Email: dansen@inbox.ru
Russian Federation, Moscow
T. A. Smirnova
Laboratory of Indication and Ultrastructural Analysis of Microorganisms, N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of Russian Federation
Email: dansen@inbox.ru
Russian Federation, Moscow
G. A. Danilina
I. M. Sechenov First Moscow Medical University, Ministry of Health of the Russian Federation
Email: dansen@inbox.ru
Russian Federation, Moscow
A. A. Adzhieva
Laboratory of Indication and Ultrastructural Analysis of Microorganisms, N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of Russian Federation
Email: dansen@inbox.ru
Russian Federation, Moscow
S. G. Andreevskaya
Laboratory of Indication and Ultrastructural Analysis of Microorganisms, N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of Russian Federation
Email: dansen@inbox.ru
Russian Federation, Moscow
N. V. Shevlyagina
Laboratory of Indication and Ultrastructural Analysis of Microorganisms, N. F. Gamaleya Federal Research Center of Epidemiology and Microbiology, Ministry of Health of Russian Federation
Email: dansen@inbox.ru
Russian Federation, Moscow
V. G. Zhukhovitsky
I. M. Sechenov First Moscow Medical University, Ministry of Health of the Russian Federation
Email: dansen@inbox.ru
Russian Federation, Moscow