In Vitro Effects of Sodium Nitroprusside and L-Nω-Nitroarginine Methyl Ester (L-NAME) on Activity of Lysosomal Cysteine Proteinases and Lysosomal Membrane Permeability
- 作者: Fomina M.1, Kudlaeva A.1, Isakov S.1, Davydov V.1
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隶属关系:
- I. P. Pavlov Ryazan State Medical University, Ministry of the Health of the Russian Federation
- 期: 卷 165, 编号 1 (2018)
- 页面: 36-39
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239951
- DOI: https://doi.org/10.1007/s10517-018-4093-y
- ID: 239951
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详细
The direct effect of 5 mM L-NAME and 0.1 mM sodium nitroprusside on activity of lysosomal cysteine proteinases and permeability of lysosomal membrane was studied in vitro after 1, 2, and 4 h of incubation. Isolated from the liver of intact female rats lysosome suspensions were used. Both substances reduced total activity of cathepsin H and did not affect cathepsin B at all time intervals. L-NAME increased cathepsin L activity at all incubation times, while sodium nitroprusside increased activity of this enzyme after 2-h incubation and reduced it incubation after 4-h incubation. L-NAME demonstrated a membrane-destabilizing effect in in vitro experiments, while sodium nitroprusside on the contrary stabilized lysosomal membranes.
作者简介
M. Fomina
I. P. Pavlov Ryazan State Medical University, Ministry of the Health of the Russian Federation
Email: anyakudlaeva@mail.ru
俄罗斯联邦, Ryazan
A. Kudlaeva
I. P. Pavlov Ryazan State Medical University, Ministry of the Health of the Russian Federation
编辑信件的主要联系方式.
Email: anyakudlaeva@mail.ru
俄罗斯联邦, Ryazan
S. Isakov
I. P. Pavlov Ryazan State Medical University, Ministry of the Health of the Russian Federation
Email: anyakudlaeva@mail.ru
俄罗斯联邦, Ryazan
V. Davydov
I. P. Pavlov Ryazan State Medical University, Ministry of the Health of the Russian Federation
Email: anyakudlaeva@mail.ru
俄罗斯联邦, Ryazan