In Vitro Effects of Sodium Nitroprusside and L-Nω-Nitroarginine Methyl Ester (L-NAME) on Activity of Lysosomal Cysteine Proteinases and Lysosomal Membrane Permeability
- Authors: Fomina M.A.1, Kudlaeva A.M.1, Isakov S.A.1, Davydov V.V.1
-
Affiliations:
- I. P. Pavlov Ryazan State Medical University, Ministry of the Health of the Russian Federation
- Issue: Vol 165, No 1 (2018)
- Pages: 36-39
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/239951
- DOI: https://doi.org/10.1007/s10517-018-4093-y
- ID: 239951
Cite item
Abstract
The direct effect of 5 mM L-NAME and 0.1 mM sodium nitroprusside on activity of lysosomal cysteine proteinases and permeability of lysosomal membrane was studied in vitro after 1, 2, and 4 h of incubation. Isolated from the liver of intact female rats lysosome suspensions were used. Both substances reduced total activity of cathepsin H and did not affect cathepsin B at all time intervals. L-NAME increased cathepsin L activity at all incubation times, while sodium nitroprusside increased activity of this enzyme after 2-h incubation and reduced it incubation after 4-h incubation. L-NAME demonstrated a membrane-destabilizing effect in in vitro experiments, while sodium nitroprusside on the contrary stabilized lysosomal membranes.
About the authors
M. A. Fomina
I. P. Pavlov Ryazan State Medical University, Ministry of the Health of the Russian Federation
Email: anyakudlaeva@mail.ru
Russian Federation, Ryazan
A. M. Kudlaeva
I. P. Pavlov Ryazan State Medical University, Ministry of the Health of the Russian Federation
Author for correspondence.
Email: anyakudlaeva@mail.ru
Russian Federation, Ryazan
S. A. Isakov
I. P. Pavlov Ryazan State Medical University, Ministry of the Health of the Russian Federation
Email: anyakudlaeva@mail.ru
Russian Federation, Ryazan
V. V. Davydov
I. P. Pavlov Ryazan State Medical University, Ministry of the Health of the Russian Federation
Email: anyakudlaeva@mail.ru
Russian Federation, Ryazan