Co-Culturing of Multipotent Mesenchymal Stromal Cells with Autological and Allogenic Lymphocytes
- 作者: Kapranov N.1, Davydova Y.1, Gal’tseva I.1, Petinati N.1, Bakshinskaitė M.2, Drize N.1, Kuz’mina L.1, Parovichnikova E.1, Savchenko V.1
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隶属关系:
- Research Center of Hematology, Ministry of Health of the Russian Federation
- Department of Immunology, Faculty of Biology, M. V. Lo-monosov Moscow State University
- 期: 卷 164, 编号 4 (2018)
- 页面: 446-452
- 栏目: Immunology and Microbiology
- URL: https://journals.rcsi.science/0007-4888/article/view/239677
- DOI: https://doi.org/10.1007/s10517-018-4009-x
- ID: 239677
如何引用文章
详细
We studied the effect of autologous and allogeneic lymphocytes on multipotent mesenchymal stromal cells in co-culture. It is shown that changes in multipotent mesenchymal stromal cells and in lymphocytes did not depend on the source of lymphocytes. Contact with lymphocytes triggers expression of HLA-DR molecules on multipotent mesenchymal stromal cells and these cells lose their immune privilege. In multipotent mesenchymal stromal cells, the relative level of expression of factors involved in immunomodulation (IDO1, PTGES, and IL-6) and expression of adhesion molecule ICAM1 increased, while expression of genes involved in the differentiation of multipotent mesenchymal stromal cells remained unchanged. Priming of multipotent mesenchymal stromal cells with IFN did not affect these changes. In turn, lymphocytes underwent activation, expression of HLA-DR increased, subpopulation composition of lymphocytes changed towards the increase in the content of naïve T cells. These findings are important for cell therapy.
作者简介
N. Kapranov
Research Center of Hematology, Ministry of Health of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
Yu. Davydova
Research Center of Hematology, Ministry of Health of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
I. Gal’tseva
Research Center of Hematology, Ministry of Health of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
N. Petinati
Research Center of Hematology, Ministry of Health of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
M. Bakshinskaitė
Department of Immunology, Faculty of Biology, M. V. Lo-monosov Moscow State University
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
N. Drize
Research Center of Hematology, Ministry of Health of the Russian Federation
编辑信件的主要联系方式.
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
L. Kuz’mina
Research Center of Hematology, Ministry of Health of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
E. Parovichnikova
Research Center of Hematology, Ministry of Health of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow
V. Savchenko
Research Center of Hematology, Ministry of Health of the Russian Federation
Email: ndrize@yandex.ru
俄罗斯联邦, Moscow