Co-Culturing of Multipotent Mesenchymal Stromal Cells with Autological and Allogenic Lymphocytes
- Authors: Kapranov N.M.1, Davydova Y.O.1, Gal’tseva I.V.1, Petinati N.A.1, Bakshinskaitė M.V.2, Drize N.I.1, Kuz’mina L.A.1, Parovichnikova E.N.1, Savchenko V.G.1
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Affiliations:
- Research Center of Hematology, Ministry of Health of the Russian Federation
- Department of Immunology, Faculty of Biology, M. V. Lo-monosov Moscow State University
- Issue: Vol 164, No 4 (2018)
- Pages: 446-452
- Section: Immunology and Microbiology
- URL: https://journals.rcsi.science/0007-4888/article/view/239677
- DOI: https://doi.org/10.1007/s10517-018-4009-x
- ID: 239677
Cite item
Abstract
We studied the effect of autologous and allogeneic lymphocytes on multipotent mesenchymal stromal cells in co-culture. It is shown that changes in multipotent mesenchymal stromal cells and in lymphocytes did not depend on the source of lymphocytes. Contact with lymphocytes triggers expression of HLA-DR molecules on multipotent mesenchymal stromal cells and these cells lose their immune privilege. In multipotent mesenchymal stromal cells, the relative level of expression of factors involved in immunomodulation (IDO1, PTGES, and IL-6) and expression of adhesion molecule ICAM1 increased, while expression of genes involved in the differentiation of multipotent mesenchymal stromal cells remained unchanged. Priming of multipotent mesenchymal stromal cells with IFN did not affect these changes. In turn, lymphocytes underwent activation, expression of HLA-DR increased, subpopulation composition of lymphocytes changed towards the increase in the content of naïve T cells. These findings are important for cell therapy.
About the authors
N. M. Kapranov
Research Center of Hematology, Ministry of Health of the Russian Federation
Email: ndrize@yandex.ru
Russian Federation, Moscow
Yu. O. Davydova
Research Center of Hematology, Ministry of Health of the Russian Federation
Email: ndrize@yandex.ru
Russian Federation, Moscow
I. V. Gal’tseva
Research Center of Hematology, Ministry of Health of the Russian Federation
Email: ndrize@yandex.ru
Russian Federation, Moscow
N. A. Petinati
Research Center of Hematology, Ministry of Health of the Russian Federation
Email: ndrize@yandex.ru
Russian Federation, Moscow
M. V. Bakshinskaitė
Department of Immunology, Faculty of Biology, M. V. Lo-monosov Moscow State University
Email: ndrize@yandex.ru
Russian Federation, Moscow
N. I. Drize
Research Center of Hematology, Ministry of Health of the Russian Federation
Author for correspondence.
Email: ndrize@yandex.ru
Russian Federation, Moscow
L. A. Kuz’mina
Research Center of Hematology, Ministry of Health of the Russian Federation
Email: ndrize@yandex.ru
Russian Federation, Moscow
E. N. Parovichnikova
Research Center of Hematology, Ministry of Health of the Russian Federation
Email: ndrize@yandex.ru
Russian Federation, Moscow
V. G. Savchenko
Research Center of Hematology, Ministry of Health of the Russian Federation
Email: ndrize@yandex.ru
Russian Federation, Moscow