Apolipoprotein A-I Stimulates Cell Proliferation in Bone Marrow Cell Culture
- 作者: Usynin I.1, Dudarev A.1, Gorodetskaya A.1, Miroshnichenko S.1, Tkachenko T.1, Tkachenko V.1
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隶属关系:
- Research Institute of Biochemistry
- 期: 卷 164, 编号 3 (2018)
- 页面: 308-311
- 栏目: Biophysics and Biochemistry
- URL: https://journals.rcsi.science/0007-4888/article/view/239570
- DOI: https://doi.org/10.1007/s10517-018-3978-0
- ID: 239570
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详细
Culturing of bone marrow cells in serum-free RPMI-1640 medium led to a decrease in the rate of DNA biosynthesis. Addition of HDL or their main protein component apolipoprotein A-I to the culture medium dose-dependently increased the rate of [3H]-thymidine incorporation into DNA. The maximum stimulation was achieved at HDL concentration of 80 μg/ml and apolipoprotein A-I concentration of 20 μg/ml. To identify the target-cells of apolipoprotein A-I, we used thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) that incorporates into cell DNA at the stage of replicative DNA synthesis (S phase) and can be detected by fluorescence microscopy. In bone marrow cell culture, apolipoprotein A-I stimulates the proliferation of monocyte (monoblasts, promonocytes) and granulocyte (myeloblasts, promyelocytes) progenitor cells, as well as bone marrow stromal cells.
作者简介
I. Usynin
Research Institute of Biochemistry
编辑信件的主要联系方式.
Email: ivan.usynin@niibch.ru
俄罗斯联邦, Novosibirsk
A. Dudarev
Research Institute of Biochemistry
Email: ivan.usynin@niibch.ru
俄罗斯联邦, Novosibirsk
A. Gorodetskaya
Research Institute of Biochemistry
Email: ivan.usynin@niibch.ru
俄罗斯联邦, Novosibirsk
S. Miroshnichenko
Research Institute of Biochemistry
Email: ivan.usynin@niibch.ru
俄罗斯联邦, Novosibirsk
T. Tkachenko
Email: ivan.usynin@niibch.ru
V. Tkachenko
Research Institute of Biochemistry
Email: ivan.usynin@niibch.ru
俄罗斯联邦, Novosibirsk