Apolipoprotein A-I Stimulates Cell Proliferation in Bone Marrow Cell Culture
- Authors: Usynin I.F.1, Dudarev A.N.1, Gorodetskaya A.Y.1, Miroshnichenko S.M.1, Tkachenko T.A.1, Tkachenko V.I.1
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Affiliations:
- Research Institute of Biochemistry
- Issue: Vol 164, No 3 (2018)
- Pages: 308-311
- Section: Biophysics and Biochemistry
- URL: https://journals.rcsi.science/0007-4888/article/view/239570
- DOI: https://doi.org/10.1007/s10517-018-3978-0
- ID: 239570
Cite item
Abstract
Culturing of bone marrow cells in serum-free RPMI-1640 medium led to a decrease in the rate of DNA biosynthesis. Addition of HDL or their main protein component apolipoprotein A-I to the culture medium dose-dependently increased the rate of [3H]-thymidine incorporation into DNA. The maximum stimulation was achieved at HDL concentration of 80 μg/ml and apolipoprotein A-I concentration of 20 μg/ml. To identify the target-cells of apolipoprotein A-I, we used thymidine analogue 5-ethynyl-2’-deoxyuridine (EdU) that incorporates into cell DNA at the stage of replicative DNA synthesis (S phase) and can be detected by fluorescence microscopy. In bone marrow cell culture, apolipoprotein A-I stimulates the proliferation of monocyte (monoblasts, promonocytes) and granulocyte (myeloblasts, promyelocytes) progenitor cells, as well as bone marrow stromal cells.
About the authors
I. F. Usynin
Research Institute of Biochemistry
Author for correspondence.
Email: ivan.usynin@niibch.ru
Russian Federation, Novosibirsk
A. N. Dudarev
Research Institute of Biochemistry
Email: ivan.usynin@niibch.ru
Russian Federation, Novosibirsk
A. Yu. Gorodetskaya
Research Institute of Biochemistry
Email: ivan.usynin@niibch.ru
Russian Federation, Novosibirsk
S. M. Miroshnichenko
Research Institute of Biochemistry
Email: ivan.usynin@niibch.ru
Russian Federation, Novosibirsk
T. A. Tkachenko
Email: ivan.usynin@niibch.ru
V. I. Tkachenko
Research Institute of Biochemistry
Email: ivan.usynin@niibch.ru
Russian Federation, Novosibirsk