Modifying Effect of Autotransfusion of Mesenchymal Stromal Cells on the Production of Reactive Oxygen Species and Cytokines by Mononuclear Cells in Patients with Chronic Heart Failure
- 作者: Petrov V.1, Agaeva E.1, Popovkina O.1, Konoplyannikov A.1, Kaplan M.1, Lepekhina L.1, Sayapina E.1, Semenkova I.1
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隶属关系:
- A. F. Tsyb Medical Radiological Research Center, Affiliated Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation
- 期: 卷 164, 编号 2 (2017)
- 页面: 233-240
- 栏目: Сell Technologies in Biology and Medicine
- URL: https://journals.rcsi.science/0007-4888/article/view/239532
- DOI: https://doi.org/10.1007/s10517-017-3965-x
- ID: 239532
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详细
We studied in vivo modifying effect of autotransfusion of human bone marrow mesenchymal stromal cells on ROS generation and production of cytokines (TNFα,TNFβ, IL-1α, IL-10, IFNγ, and GM-CSF) and PGE2 by mononuclear cells of patients (N=21) with chronic heart failure. These parameters were evaluated prior to (control) and after (immediately and on day 14) intravenous administration of stromal cells in doses of 100-200×106. Immediately after autotransfusion, significant increase of in vitro zymosan-induced chemiluminescence of blood mononuclear cells from 10 patients was observed. At later terms after autotransfusion (day 14), inhibition of chemiluminescent activity of blood mononuclear cells was revealed in 50% patients. We discuss possible mechanisms of involvement of transplanted autologous bone marrow mesenchymal stromal cells in reprogramming of blood mononuclear phagocytes from the pro- to anti-inflammatory phenotype under conditions of their in vivo interaction manifesting in transition from activation to inhibition of ROS-producing activity of macrophages and significant suppression of in vitro LPS-induced production of TNFα and GM-CSF by blood mononuclears against the background of significantly elevated TNFβ, IL-10, and IL-1α concentrations.
作者简介
V. Petrov
A. F. Tsyb Medical Radiological Research Center, Affiliated Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: ekagaeva@eandex.ru
俄罗斯联邦, Obninsk
E. Agaeva
A. F. Tsyb Medical Radiological Research Center, Affiliated Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation
编辑信件的主要联系方式.
Email: ekagaeva@eandex.ru
俄罗斯联邦, Obninsk
O. Popovkina
A. F. Tsyb Medical Radiological Research Center, Affiliated Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: ekagaeva@eandex.ru
俄罗斯联邦, Obninsk
A. Konoplyannikov
A. F. Tsyb Medical Radiological Research Center, Affiliated Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: ekagaeva@eandex.ru
俄罗斯联邦, Obninsk
M. Kaplan
A. F. Tsyb Medical Radiological Research Center, Affiliated Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: ekagaeva@eandex.ru
俄罗斯联邦, Obninsk
L. Lepekhina
A. F. Tsyb Medical Radiological Research Center, Affiliated Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: ekagaeva@eandex.ru
俄罗斯联邦, Obninsk
E. Sayapina
A. F. Tsyb Medical Radiological Research Center, Affiliated Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: ekagaeva@eandex.ru
俄罗斯联邦, Obninsk
I. Semenkova
A. F. Tsyb Medical Radiological Research Center, Affiliated Branch of the National Medical Research Radiological Center, Ministry of Health of the Russian Federation
Email: ekagaeva@eandex.ru
俄罗斯联邦, Obninsk