Human Umbilical Cord Mesenchymal Stromal Cells Support Viability of Umbilical Cord Blood Hematopoietic Stem Cells but not the “Stemness” of Their Progeny in Co-Culture
- 作者: Romanov Y.1,2, Volgina N.3, Balashova E.2, Kabaeva N.1, Dugina T.2, Sukhikh G.3
-
隶属关系:
- Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation
- CryoCenter Cord Blood Bank
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- 期: 卷 163, 编号 4 (2017)
- 页面: 523-527
- 栏目: Cell Technologies in Biology and Medicine
- URL: https://journals.rcsi.science/0007-4888/article/view/239072
- DOI: https://doi.org/10.1007/s10517-017-3843-6
- ID: 239072
如何引用文章
详细
Cell—cell interactions and the ability of mesenchymal stromal cells to support the expansion of hematopoietic progenitor cells were studied in co-culture of human umbilical cord tissue-derived mesenchymal stromal cells and nucleated umbilical cord blood cells. It was found that hematopoietic stem cells from the umbilical cord blood are capable to adhere to mesenchymal stromal cells and proliferate during 3-4 weeks in co-culture. However, despite the formation of hematopoietic foci and accumulation of CD34+ and CD133+ cells in the adherent cell fraction, the ability of newly generated blood cells to form colonies in semi-solid culture medium was appreciably reduced. These findings suggest that human umbilical cord tissue-derived mesenchymal stromal cells display a weak capability to support the “stemness” of hematopoietic stem cell progeny despite long-term maintenance of their viability and proliferation.
作者简介
Yu. Romanov
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation; CryoCenter Cord Blood Bank
编辑信件的主要联系方式.
Email: romanov@cardio.ru
俄罗斯联邦, Moscow; Moscow
N. Volgina
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
俄罗斯联邦, Moscow
E. Balashova
CryoCenter Cord Blood Bank
Email: romanov@cardio.ru
俄罗斯联邦, Moscow
N. Kabaeva
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
俄罗斯联邦, Moscow
T. Dugina
CryoCenter Cord Blood Bank
Email: romanov@cardio.ru
俄罗斯联邦, Moscow
G. Sukhikh
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
俄罗斯联邦, Moscow