Human Umbilical Cord Mesenchymal Stromal Cells Support Viability of Umbilical Cord Blood Hematopoietic Stem Cells but not the “Stemness” of Their Progeny in Co-Culture
- Авторлар: Romanov Y.1,2, Volgina N.3, Balashova E.2, Kabaeva N.1, Dugina T.2, Sukhikh G.3
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Мекемелер:
- Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation
- CryoCenter Cord Blood Bank
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Шығарылым: Том 163, № 4 (2017)
- Беттер: 523-527
- Бөлім: Cell Technologies in Biology and Medicine
- URL: https://journals.rcsi.science/0007-4888/article/view/239072
- DOI: https://doi.org/10.1007/s10517-017-3843-6
- ID: 239072
Дәйексөз келтіру
Аннотация
Cell—cell interactions and the ability of mesenchymal stromal cells to support the expansion of hematopoietic progenitor cells were studied in co-culture of human umbilical cord tissue-derived mesenchymal stromal cells and nucleated umbilical cord blood cells. It was found that hematopoietic stem cells from the umbilical cord blood are capable to adhere to mesenchymal stromal cells and proliferate during 3-4 weeks in co-culture. However, despite the formation of hematopoietic foci and accumulation of CD34+ and CD133+ cells in the adherent cell fraction, the ability of newly generated blood cells to form colonies in semi-solid culture medium was appreciably reduced. These findings suggest that human umbilical cord tissue-derived mesenchymal stromal cells display a weak capability to support the “stemness” of hematopoietic stem cell progeny despite long-term maintenance of their viability and proliferation.
Авторлар туралы
Yu. Romanov
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation; CryoCenter Cord Blood Bank
Хат алмасуға жауапты Автор.
Email: romanov@cardio.ru
Ресей, Moscow; Moscow
N. Volgina
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
Ресей, Moscow
E. Balashova
CryoCenter Cord Blood Bank
Email: romanov@cardio.ru
Ресей, Moscow
N. Kabaeva
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
Ресей, Moscow
T. Dugina
CryoCenter Cord Blood Bank
Email: romanov@cardio.ru
Ресей, Moscow
G. Sukhikh
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
Ресей, Moscow