Effect of Concentration of Collagen Gel on Functional Activity of Bone Marrow Mesenchymal Stromal Cells
- 作者: Nashchekina Y.1,2, Yudintceva N.1, Nikonov P.1, Ivanova E.1, Smagina L.1, Voronkina I.1
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隶属关系:
- Institute of Cytology, Russian Academy of Sciences
- Peter the Great St. Petersburg State Polytechnic University
- 期: 卷 163, 编号 1 (2017)
- 页面: 123-128
- 栏目: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/238702
- DOI: https://doi.org/10.1007/s10517-017-3751-9
- ID: 238702
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详细
Collagen I gels with protein concentrations of 1, 2, and 3.5 mg/ml were prepared and embedded in a porous polylactide scaffold to reduce their contraction. Concentration of the gel did not affect its degradation. Collagen gels promoted the formation of cell networks. The cells in the collagen gel with a concentration of 1 mg/ml embedded in polylactide scaffold had elongated spindle-like shape, in contrast to flattened cells in collagen gel of the same concentration not embedded in the scaffold. Stabilization of the collagen gel in the polylactide scaffold promoted active synthesis of laminin and fibronectin by cells as soon as on day 5 of culturing in comparison with that in free collagen substrate.
作者简介
Yu. Nashchekina
Institute of Cytology, Russian Academy of Sciences; Peter the Great St. Petersburg State Polytechnic University
编辑信件的主要联系方式.
Email: yuliya.shved@gmail.com
俄罗斯联邦, St. Petersburg; St. Petersburg
N. Yudintceva
Institute of Cytology, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
俄罗斯联邦, St. Petersburg
P. Nikonov
Institute of Cytology, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
俄罗斯联邦, St. Petersburg
E. Ivanova
Institute of Cytology, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
俄罗斯联邦, St. Petersburg
L. Smagina
Institute of Cytology, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
俄罗斯联邦, St. Petersburg
I. Voronkina
Institute of Cytology, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
俄罗斯联邦, St. Petersburg
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