Effect of Concentration of Collagen Gel on Functional Activity of Bone Marrow Mesenchymal Stromal Cells
- Authors: Nashchekina Y.A.1,2, Yudintceva N.M.1, Nikonov P.O.1, Ivanova E.A.1, Smagina L.V.1, Voronkina I.V.1
-
Affiliations:
- Institute of Cytology, Russian Academy of Sciences
- Peter the Great St. Petersburg State Polytechnic University
- Issue: Vol 163, No 1 (2017)
- Pages: 123-128
- Section: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/238702
- DOI: https://doi.org/10.1007/s10517-017-3751-9
- ID: 238702
Cite item
Abstract
Collagen I gels with protein concentrations of 1, 2, and 3.5 mg/ml were prepared and embedded in a porous polylactide scaffold to reduce their contraction. Concentration of the gel did not affect its degradation. Collagen gels promoted the formation of cell networks. The cells in the collagen gel with a concentration of 1 mg/ml embedded in polylactide scaffold had elongated spindle-like shape, in contrast to flattened cells in collagen gel of the same concentration not embedded in the scaffold. Stabilization of the collagen gel in the polylactide scaffold promoted active synthesis of laminin and fibronectin by cells as soon as on day 5 of culturing in comparison with that in free collagen substrate.
About the authors
Yu. A. Nashchekina
Institute of Cytology, Russian Academy of Sciences; Peter the Great St. Petersburg State Polytechnic University
Author for correspondence.
Email: yuliya.shved@gmail.com
Russian Federation, St. Petersburg; St. Petersburg
N. M. Yudintceva
Institute of Cytology, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
Russian Federation, St. Petersburg
P. O. Nikonov
Institute of Cytology, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
Russian Federation, St. Petersburg
E. A. Ivanova
Institute of Cytology, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
Russian Federation, St. Petersburg
L. V. Smagina
Institute of Cytology, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
Russian Federation, St. Petersburg
I. V. Voronkina
Institute of Cytology, Russian Academy of Sciences
Email: yuliya.shved@gmail.com
Russian Federation, St. Petersburg