Human Umbilical Cord Blood Serum: Effective Substitute of Fetal Bovine Serum for Culturing of Human Multipotent Mesenchymal Stromal Cells
- 作者: Romanov Y.1,2, Balashova E.1,2, Volgina N.3, Kabaeva N.1, Dugina T.2, Sukhikh G.3
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隶属关系:
- Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation
- CryoCenter Cord Blood Bank
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- 期: 卷 162, 编号 4 (2017)
- 页面: 528-533
- 栏目: Cell Technologies in Biology and Medicine
- URL: https://journals.rcsi.science/0007-4888/article/view/238352
- DOI: https://doi.org/10.1007/s10517-017-3654-9
- ID: 238352
如何引用文章
详细
Optimal conditions for culturing of multipotent mesenchymal stromal cells in the presence of pooled umbilical cord blood serum were determined. It was found that umbilical cord blood serum in a concentration range of 1-10% effectively supported high viability and proliferative activity of cells with unaltered phenotype and preserved multilineage differentiation capacity. The proposed approach allows avoiding the use of xenogenic animal sera for culturing of multipotent mesenchymal stromal cells and creates prerequisites for designing and manufacturing safe cellular and/or acellular products for medical purposes.
作者简介
Yu. Romanov
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation; CryoCenter Cord Blood Bank
编辑信件的主要联系方式.
Email: romanov@cardio.ru
俄罗斯联邦, Moscow; Moscow
E. Balashova
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation; CryoCenter Cord Blood Bank
Email: romanov@cardio.ru
俄罗斯联邦, Moscow; Moscow
N. Volgina
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
俄罗斯联邦, Moscow
N. Kabaeva
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
俄罗斯联邦, Moscow
T. Dugina
CryoCenter Cord Blood Bank
Email: romanov@cardio.ru
俄罗斯联邦, Moscow
G. Sukhikh
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
俄罗斯联邦, Moscow