Human Umbilical Cord Blood Serum: Effective Substitute of Fetal Bovine Serum for Culturing of Human Multipotent Mesenchymal Stromal Cells
- Авторлар: Romanov Y.1,2, Balashova E.1,2, Volgina N.3, Kabaeva N.1, Dugina T.2, Sukhikh G.3
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Мекемелер:
- Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation
- CryoCenter Cord Blood Bank
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Шығарылым: Том 162, № 4 (2017)
- Беттер: 528-533
- Бөлім: Cell Technologies in Biology and Medicine
- URL: https://journals.rcsi.science/0007-4888/article/view/238352
- DOI: https://doi.org/10.1007/s10517-017-3654-9
- ID: 238352
Дәйексөз келтіру
Аннотация
Optimal conditions for culturing of multipotent mesenchymal stromal cells in the presence of pooled umbilical cord blood serum were determined. It was found that umbilical cord blood serum in a concentration range of 1-10% effectively supported high viability and proliferative activity of cells with unaltered phenotype and preserved multilineage differentiation capacity. The proposed approach allows avoiding the use of xenogenic animal sera for culturing of multipotent mesenchymal stromal cells and creates prerequisites for designing and manufacturing safe cellular and/or acellular products for medical purposes.
Авторлар туралы
Yu. Romanov
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation; CryoCenter Cord Blood Bank
Хат алмасуға жауапты Автор.
Email: romanov@cardio.ru
Ресей, Moscow; Moscow
E. Balashova
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation; CryoCenter Cord Blood Bank
Email: romanov@cardio.ru
Ресей, Moscow; Moscow
N. Volgina
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
Ресей, Moscow
N. Kabaeva
Russian Cardiology Research-and-Production Complex, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
Ресей, Moscow
T. Dugina
CryoCenter Cord Blood Bank
Email: romanov@cardio.ru
Ресей, Moscow
G. Sukhikh
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: romanov@cardio.ru
Ресей, Moscow