Artifacts Arising from Using Leukocytic Fc Receptor Blocking Buffer
- 作者: Zubova S.1, Kabanov D.1, Serov D.1, Grachev S.1, Prokhorenko I.1
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隶属关系:
- Institute of Fundamental Problems of Biology, Russian Academy of Sciences
- 期: 卷 162, 编号 2 (2016)
- 页面: 244-247
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238082
- DOI: https://doi.org/10.1007/s10517-016-3586-9
- ID: 238082
如何引用文章
详细
We studied the effects of Human TruStain FcX buffer (Fcγ receptor blocking solution) in experiments on evaluation of TLR4 level with labeled monoclonal antibodies, intracellular immunofluorescent staining of NF-κB p50, and TNF-α synthesis on human isolated monocytes and whole blood cells. The influence of the blocking buffer on the measured parameters should be taken into account and appropriateness of its use in experiments on isolated cells and whole blood should be considered.
作者简介
S. Zubova
Institute of Fundamental Problems of Biology, Russian Academy of Sciences
编辑信件的主要联系方式.
Email: zusvet@rambler.ru
俄罗斯联邦, Pushchino
D. Kabanov
Institute of Fundamental Problems of Biology, Russian Academy of Sciences
Email: zusvet@rambler.ru
俄罗斯联邦, Pushchino
D. Serov
Institute of Fundamental Problems of Biology, Russian Academy of Sciences
Email: zusvet@rambler.ru
俄罗斯联邦, Pushchino
S. Grachev
Institute of Fundamental Problems of Biology, Russian Academy of Sciences
Email: zusvet@rambler.ru
俄罗斯联邦, Pushchino
I. Prokhorenko
Institute of Fundamental Problems of Biology, Russian Academy of Sciences
Email: zusvet@rambler.ru
俄罗斯联邦, Pushchino