Artifacts Arising from Using Leukocytic Fc Receptor Blocking Buffer
- Authors: Zubova S.V.1, Kabanov D.S.1, Serov D.A.1, Grachev S.V.1, Prokhorenko I.R.1
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Affiliations:
- Institute of Fundamental Problems of Biology, Russian Academy of Sciences
- Issue: Vol 162, No 2 (2016)
- Pages: 244-247
- Section: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238082
- DOI: https://doi.org/10.1007/s10517-016-3586-9
- ID: 238082
Cite item
Abstract
We studied the effects of Human TruStain FcX buffer (Fcγ receptor blocking solution) in experiments on evaluation of TLR4 level with labeled monoclonal antibodies, intracellular immunofluorescent staining of NF-κB p50, and TNF-α synthesis on human isolated monocytes and whole blood cells. The influence of the blocking buffer on the measured parameters should be taken into account and appropriateness of its use in experiments on isolated cells and whole blood should be considered.
About the authors
S. V. Zubova
Institute of Fundamental Problems of Biology, Russian Academy of Sciences
Author for correspondence.
Email: zusvet@rambler.ru
Russian Federation, Pushchino
D. S. Kabanov
Institute of Fundamental Problems of Biology, Russian Academy of Sciences
Email: zusvet@rambler.ru
Russian Federation, Pushchino
D. A. Serov
Institute of Fundamental Problems of Biology, Russian Academy of Sciences
Email: zusvet@rambler.ru
Russian Federation, Pushchino
S. V. Grachev
Institute of Fundamental Problems of Biology, Russian Academy of Sciences
Email: zusvet@rambler.ru
Russian Federation, Pushchino
I. R. Prokhorenko
Institute of Fundamental Problems of Biology, Russian Academy of Sciences
Email: zusvet@rambler.ru
Russian Federation, Pushchino