Effects of Humic Acids Isolated from Peat of Various Origin on in Vitro Production of Nitric Oxide: a Screening Study
- 作者: Trofimova E.1, Zykova M.2, Ligacheva A.1, Sherstoboev E.1, Zhdanov V.1, Belousov M.2, Yusubov M.2, Krivoshchekov S.2, Danilets M.1, Dygai A.1
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隶属关系:
- E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
- Siberian State Medical University
- 期: 卷 161, 编号 5 (2016)
- 页面: 687-692
- 栏目: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/237679
- DOI: https://doi.org/10.1007/s10517-016-3486-z
- ID: 237679
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详细
A screening study of biological activity of native humic acids isolated from peat was performed; several physical and chemical parameters of their structures were studied by UV- and infrared spectroscopy. Spectroscopy yielded similar shape of light absorption curves of humic acids of different origin, which can reflect similarity of general structural principles of these substances. Alkaline humic acids have more developed system of polyconjugation, while molecular structures of pyrophosphate humic acids were characterized by higher aromaticity and condensation indexes. Biological activity of the studied humic acids was assessed by NO-stimulating capacity during their culturing with murine peritoneal macrophages in a wide concentration range. It was shown that due to dose-dependent enhancement of NO production humic acids can change the functional state of macrophages towards development of pro-inflammatory properties. These changes were associated with high activity of humic acids isolated by pyrophosphate extraction, which allows considering effects of isolation method on biological activity.
作者简介
E. Trofimova
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
M. Zykova
Siberian State Medical University
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
A. Ligacheva
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
E. Sherstoboev
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
V. Zhdanov
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
M. Belousov
Siberian State Medical University
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
M. Yusubov
Siberian State Medical University
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
S. Krivoshchekov
Siberian State Medical University
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
M. Danilets
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
编辑信件的主要联系方式.
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
A. Dygai
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk