Effects of Water-Soluble Polysaccharides with Different Chemical Structure, Isolated from Acorus calamus L. and Trifolium pratense L., on Nitric Oxide Production: A Screening Study
- 作者: Ligacheva A.1, Danilets M.1, mova E.1, Sherstoboev E.1, Zhdanov V.1, Guriev A.2, Belousov M.2, Yusubov M.2,3, Korzh A.2, Krivoshchekov S.2,3, Dygai A.1
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隶属关系:
- E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
- Siberian State Medical University
- National Research Tomsk Polytechnological University
- 期: 卷 160, 编号 3 (2016)
- 页面: 330-335
- 栏目: Pharmacology and Toxicology
- URL: https://journals.rcsi.science/0007-4888/article/view/236699
- DOI: https://doi.org/10.1007/s10517-016-3163-2
- ID: 236699
如何引用文章
详细
Screening study of the effects of sweet flag (Acorus calamus L.) rhizome and clover (Trifolium pratense L.) aerial part on the production of NO by mouse macrophages was carried out. The polysaccharides were separated by ion exchange chromatography into fractions differing by monomeric composition and ramification type and were used in concentrations of 20, 40, and 100 μg/ml. Four fractions of Acorus calamus L. (PSF-101, PSF-102, PSF-103, and PSF-105), used in different concentrations, moderately stimulated nitrite production by macrophages. Three of five Trifolium pratense L. polysaccharides (PS62-3, PS62-4, and PS62-5) exhibited a significant specific effect on NO production. Rhamnogalactouronans from clover PS63-3 in all concentrations and from PS62-5 in a concentration of 100 μg/ml exhibited the highest activity, comparable to the NO-stimulatory activity of the reference LPS, while polysaccharide PS62-3 in a concentration of 40 μg/ml exhibited even higher activity.
作者简介
A. Ligacheva
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
M. Danilets
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
编辑信件的主要联系方式.
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
E. mova
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
E. Sherstoboev
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
V. Zhdanov
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
A. Guriev
Siberian State Medical University
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
M. Belousov
Siberian State Medical University
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
M. Yusubov
Siberian State Medical University; National Research Tomsk Polytechnological University
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk; Tomsk
A. Korzh
Siberian State Medical University
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk
S. Krivoshchekov
Siberian State Medical University; National Research Tomsk Polytechnological University
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk; Tomsk
A. Dygai
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: m.danilets@mail.ru
俄罗斯联邦, Tomsk