Isolation of IgG Associated with Human Placenta
- Авторы: Ignat’eva N.1, Ziganshina M.1, Shilova N.2, Khasbiullina N.2, Bovin N.2, Tyutyunnik V.1, Sukhikh G.1
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Учреждения:
- V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Выпуск: Том 167, № 1 (2019)
- Страницы: 120-122
- Раздел: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241503
- DOI: https://doi.org/10.1007/s10517-019-04474-4
- ID: 241503
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Аннотация
We optimized the method of isolation of antibodies from placental tissue of a conventionally healthy patient. Four protocols of antibody isolation were evaluated and a protocol with tissue grinding (without homogenization) and successive elution of the antibodies with acidic and alkaline buffers was recommended for use. The repertoire of the isolated antibodies was characterized using a glycan array. Partial coincidence of the specificity of the isolated antibodies with antibodies in the peripheral blood was demonstrated, which indicates their possible association with carbohydrate antigens in the placenta. Identification of potential molecular targets of resident antibodies in the placenta is necessary for understanding the mechanisms of formation of immunological tolerance to the fetus.
Об авторах
N. Ignat’eva
V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: mmz@mail.ru
Россия, Moscow
M. Ziganshina
V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Автор, ответственный за переписку.
Email: mmz@mail.ru
Россия, Moscow
N. Shilova
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: mmz@mail.ru
Россия, Moscow
N. Khasbiullina
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: mmz@mail.ru
Россия, Moscow
N. Bovin
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: mmz@mail.ru
Россия, Moscow
V. Tyutyunnik
V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: mmz@mail.ru
Россия, Moscow
G. Sukhikh
V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: mmz@mail.ru
Россия, Moscow
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