Isolation of IgG Associated with Human Placenta
- Autores: Ignat’eva N.1, Ziganshina M.1, Shilova N.2, Khasbiullina N.2, Bovin N.2, Tyutyunnik V.1, Sukhikh G.1
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Afiliações:
- V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Edição: Volume 167, Nº 1 (2019)
- Páginas: 120-122
- Seção: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/241503
- DOI: https://doi.org/10.1007/s10517-019-04474-4
- ID: 241503
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Resumo
We optimized the method of isolation of antibodies from placental tissue of a conventionally healthy patient. Four protocols of antibody isolation were evaluated and a protocol with tissue grinding (without homogenization) and successive elution of the antibodies with acidic and alkaline buffers was recommended for use. The repertoire of the isolated antibodies was characterized using a glycan array. Partial coincidence of the specificity of the isolated antibodies with antibodies in the peripheral blood was demonstrated, which indicates their possible association with carbohydrate antigens in the placenta. Identification of potential molecular targets of resident antibodies in the placenta is necessary for understanding the mechanisms of formation of immunological tolerance to the fetus.
Sobre autores
N. Ignat’eva
V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: mmz@mail.ru
Rússia, Moscow
M. Ziganshina
V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Autor responsável pela correspondência
Email: mmz@mail.ru
Rússia, Moscow
N. Shilova
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: mmz@mail.ru
Rússia, Moscow
N. Khasbiullina
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: mmz@mail.ru
Rússia, Moscow
N. Bovin
M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: mmz@mail.ru
Rússia, Moscow
V. Tyutyunnik
V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: mmz@mail.ru
Rússia, Moscow
G. Sukhikh
V. I. Kulakov National Medical Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: mmz@mail.ru
Rússia, Moscow