Application of Adeno-Associated Virus Vectors for Engineering SCF-Containing Extracellular Vesicles of Mesenchymal Stromal Cells
- Авторы: Zubkova E.1,2, Beloglazova I.1,2, Evtushenko E.1, Kopylov A.1, Shevchenko E.1, Dergilev K.1,2, Ratner E.1, Parfenova E.1,2, Men’shikov M.1
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Учреждения:
- National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation
- M. V. Lomonosov Moscow State University
- Выпуск: Том 166, № 4 (2019)
- Страницы: 527-534
- Раздел: Cell Technologies in Biology and Medicine
- URL: https://journals.rcsi.science/0007-4888/article/view/241158
- DOI: https://doi.org/10.1007/s10517-019-04387-2
- ID: 241158
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Аннотация
Mesenchymal stromal cells from rat adipose tissue were transduced with adeno-associated viral (AAV) vector encoding stem cell factor SCF that stimulates proliferation of cardiac c-kit+ cells and improved cardiac function and survival of animals after myocardial infarction. Extracellular vesicles isolated from the medium conditioned by mesenchymal stromal cells by ultracentrifugation were characterized by Western blotting, transmission electron microscopy, nanoparticle tracking analysis, immunostaining, and mass spectrometry analysis. Using proteomic analysis, we identified transgenic SCF in extracellular vesicles released by AAV-modified mesenchymal stromal cells and detected some proteins specific of extracellular vesicles secreted by transduced cells. Extracellular vesicles from AAV-transduced mesenchymal stromal cells could be used for delivery of transgenic proteins as they were readily endocytosed by both cardiosphere-derived cells and cardiac-progenitor cells.
Об авторах
E. Zubkova
National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation; M. V. Lomonosov Moscow State University
Автор, ответственный за переписку.
Email: cat.zubkova@gmail.com
Россия, Moscow; Moscow
I. Beloglazova
National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation; M. V. Lomonosov Moscow State University
Email: cat.zubkova@gmail.com
Россия, Moscow; Moscow
E. Evtushenko
National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation
Email: cat.zubkova@gmail.com
Россия, Moscow
A. Kopylov
Email: cat.zubkova@gmail.com
E. Shevchenko
National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation
Email: cat.zubkova@gmail.com
Россия, Moscow
K. Dergilev
National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation; M. V. Lomonosov Moscow State University
Email: cat.zubkova@gmail.com
Россия, Moscow; Moscow
E. Ratner
National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation
Email: cat.zubkova@gmail.com
Россия, Moscow
E. Parfenova
National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation; M. V. Lomonosov Moscow State University
Email: cat.zubkova@gmail.com
Россия, Moscow; Moscow
M. Men’shikov
National Medical Research Center for Cardiology, Ministry of Health of the Russian Federation
Email: cat.zubkova@gmail.com
Россия, Moscow