Application of Impedance Spectroscopy for the Control of the Integrity of In Vitro Models of Barrier Tissues


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Аннотация

We compared available methods for monitoring the integrity of in vitro models of barrier tissues and studied the possibility of using impedance spectroscopy to solve this problem. It was demonstrated (theoretically and experimentally) that TEER measurements are not sufficiently sensitive to detect small defects in the cell barrier that significantly affect its permeability. For obtaining reliable results, it is necessary to set a sufficiently high threshold TEER, which leads to the loss of many intact samples. At the same time, impedance spectroscopy has all advantages of the classical method of measuring TEER (it is rapid and non-invasive method), while its application in combination with the methods of machine learning allows reliable detection of defects in the cell barrier.

Об авторах

S. Nikulin

BioClinicum Research Center, Ministry of Health of the Russian Federation; Moscow Institute of Physics and Technology (MIPT)

Автор, ответственный за переписку.
Email: brazor@inbox.ru
Россия, Obninsk; Moscow

T. Gerasimenko

BioClinicum Research Center, Ministry of Health of the Russian Federation

Email: brazor@inbox.ru
Россия, Obninsk

S. Shilin

BioClinicum Research Center, Ministry of Health of the Russian Federation

Email: brazor@inbox.ru
Россия, Obninsk

G. Zakharova

BioClinicum Research Center, Ministry of Health of the Russian Federation

Email: brazor@inbox.ru
Россия, Obninsk

I. Gazizov

BioClinicum Research Center, Ministry of Health of the Russian Federation

Email: brazor@inbox.ru
Россия, Obninsk

A. Poloznikov

National Research Center of Radiology, Ministry of Health of the Russian Federation

Email: brazor@inbox.ru
Россия, Obninsk

D. Sakharov

BioClinicum Research Center, Ministry of Health of the Russian Federation

Email: brazor@inbox.ru
Россия, Obninsk


© Springer Science+Business Media, LLC, part of Springer Nature, 2019

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