The Influence of Proinflammatory Factors on the Neuroprotective Efficiency of Multipotent Mesenchymal Stromal Cells in Traumatic Brain Injury
- Авторы: Danilina T.1, Silachev D.2,3, Pevzner I.2,3, Gulyaev M.4, Pirogov Y.4, Zorova L.2,3,5, Plotnikov E.2,3, Sukhikh G.3, Zorov D.2,3
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Учреждения:
- Faculty of Bioengineering and Bioinformatics, M. V. Lomonosov Moscow State University
- A. N. Belozersky Institute of Physicochemical Biology, M. V. Lomonosov Moscow State University
- V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
- Laboratory of Magnetic Tomography and Spectroscopy, Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University
- International Laser Research Center, M. V. Lomonosov Moscow State University
- Выпуск: Том 163, № 4 (2017)
- Страницы: 528-534
- Раздел: Translated from Kletochnye Tekhnologii v Biologii i Meditsine (Cell Technologies in Biology and Medicine)
- URL: https://journals.rcsi.science/0007-4888/article/view/239074
- DOI: https://doi.org/10.1007/s10517-017-3844-5
- ID: 239074
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Аннотация
We studied the neuroprotective potential of multipotent mesenchymal stromal cells in traumatic brain injury and the effect of inflammatory preconditioning on neuroprotective properties of stem cells under in vitro conditions. To this end, the effects of cell incubation with LPS or their co-culturing with leukocytes on production of cytokines IL-1α, IL-6, TNFα, and MMP-2 and MMP-9 by these cells were evaluated. Culturing under conditions simulating inflammation increased the production of all these factors by multipotent mesenchymal stromal cells. However, acquisition of the inflammatory phenotype by stromal cells did not reduce their therapeutic effectiveness in traumatic brain injury. Moreover, in some variants of inflammatory preconditioning, multipotent mesenchymal stromal cells exhibited more pronounced neuroprotective properties reducing the volume of brain lesion and promoting recovery of neurological functions after traumatic brain injury.
Об авторах
T. Danilina
Faculty of Bioengineering and Bioinformatics, M. V. Lomonosov Moscow State University
Email: plotnikov@genebee.msu.ru
Россия, Moscow
D. Silachev
A. N. Belozersky Institute of Physicochemical Biology, M. V. Lomonosov Moscow State University; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: plotnikov@genebee.msu.ru
Россия, Moscow; Moscow
I. Pevzner
A. N. Belozersky Institute of Physicochemical Biology, M. V. Lomonosov Moscow State University; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: plotnikov@genebee.msu.ru
Россия, Moscow; Moscow
M. Gulyaev
Laboratory of Magnetic Tomography and Spectroscopy, Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University
Email: plotnikov@genebee.msu.ru
Россия, Moscow
Yu. Pirogov
Laboratory of Magnetic Tomography and Spectroscopy, Faculty of Fundamental Medicine, M. V. Lomonosov Moscow State University
Email: plotnikov@genebee.msu.ru
Россия, Moscow
L. Zorova
A. N. Belozersky Institute of Physicochemical Biology, M. V. Lomonosov Moscow State University; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation; International Laser Research Center, M. V. Lomonosov Moscow State University
Email: plotnikov@genebee.msu.ru
Россия, Moscow; Moscow; Moscow
E. Plotnikov
A. N. Belozersky Institute of Physicochemical Biology, M. V. Lomonosov Moscow State University; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Автор, ответственный за переписку.
Email: plotnikov@genebee.msu.ru
Россия, Moscow; Moscow
G. Sukhikh
V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: plotnikov@genebee.msu.ru
Россия, Moscow
D. Zorov
A. N. Belozersky Institute of Physicochemical Biology, M. V. Lomonosov Moscow State University; V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation
Email: plotnikov@genebee.msu.ru
Россия, Moscow; Moscow