Genetic Engineering of Native Chain Combinations of B-Cell Repertoires on the Surface of Methylotrophic Yeasts Pichia pastoris
- Авторы: Bobik T.V.1, Shurdova E.M.1, Smirnov I.V.1, Ponomarenko N.A.1, Khurs E.N.2, Knorre V.D.1, Gabibov A.G.1
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Учреждения:
- Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
- Laboratory of Chemical Bases and Biocatalysis V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
- Выпуск: Том 163, № 2 (2017)
- Страницы: 263-267
- Раздел: Biotechnology
- URL: https://journals.rcsi.science/0007-4888/article/view/238807
- DOI: https://doi.org/10.1007/s10517-017-3780-4
- ID: 238807
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Аннотация
We designed genetic constructs for exposing Fab-fragment library of natively paired single cell B-cell receptors on the surface of Pichia pastoris yeast cells. We have previously obtained the A17 antibody in our laboratory [6]. In this study we showed that the newly designed genetic constructs provide a compatible level of A17 antibody Fab fragment on the surface of yeast cells as well as in the case of vectors containing DNA fragments corresponding to each chain of the antibody. The data suggest that the developed approach for constructing immunoglobulin gene libraries is adequate and fully convenient for studying properties of the real human B-lymphocyte repertoire.
Об авторах
T. Bobik
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: gabibov@mx.ibch.ru
Россия, Moscow
E. Shurdova
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: gabibov@mx.ibch.ru
Россия, Moscow
I. Smirnov
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: gabibov@mx.ibch.ru
Россия, Moscow
N. Ponomarenko
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: gabibov@mx.ibch.ru
Россия, Moscow
E. Khurs
Laboratory of Chemical Bases and Biocatalysis V. A. Engelhardt Institute of Molecular Biology, Russian Academy of Sciences
Email: gabibov@mx.ibch.ru
Россия, Moscow
V. Knorre
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Email: gabibov@mx.ibch.ru
Россия, Moscow
A. Gabibov
Laboratory of Biocatalysis, M. M. Shemyakin and Yu. A. Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences
Автор, ответственный за переписку.
Email: gabibov@mx.ibch.ru
Россия, Moscow
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