Regenerative Potential of Spermatogonial Stem Cells, Endothelial Progenitor Cells, and Epithelial Progenitor Cells of C57Bl/6 Male Mice with Metabolic Disorders
- Авторы: Skurikhin E.1, Pakhomova A.1, Pershina O.1, Ermolaeva L.1, Krupin V.1, Ermakova N.1, Pan E.1, Kudryashova A.1, Rybalkina O.1, Pavlovskaya T.1, Litvyakov N.2, Goldberg V.2, Dygai A.1
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Учреждения:
- E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
- Tomsk National Research Medical Center, Russian Academy of Sciences
- Выпуск: Том 163, № 2 (2017)
- Страницы: 239-244
- Раздел: Article
- URL: https://journals.rcsi.science/0007-4888/article/view/238785
- DOI: https://doi.org/10.1007/s10517-017-3775-1
- ID: 238785
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Аннотация
The properties of spermatogonial stem cells, endothelial progenitor cells, and the epithelial progenitors of C57Bl/6 mice under conditions of metabolic disorders were studied using the model of busulfan-induced suppression of spermatogenesis and in vitro culture technique. Spermatogonial stem cells CD117—CD90+ and epithelial progenitors CD45—CD31—Sca-1+CD49f+ derived from the testes of mice with metabolic disturbances demonstrated 17- and 28-fold increase in the respective cell mass and generated cell colonies in vitro. In contrast, spermatogonial stem cells with immune phenotype CD51—CD24+CD52+ had reduced selfrenewal capacity. Spermatogonial stem cells CD117—CD90+ and CD117+CD90+ as well as endothelial progenitors CD45—CD31+ derived from the testes of donor mice with metabolic disorders demonstrated high transplantation capacity in C57Bl/6 mouse testes damaged by cytostatic busulfan.
Об авторах
E. Skurikhin
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: angelinapakhomova2011@gmail.com
Россия, Tomsk
A. Pakhomova
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Автор, ответственный за переписку.
Email: angelinapakhomova2011@gmail.com
Россия, Tomsk
O. Pershina
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: angelinapakhomova2011@gmail.com
Россия, Tomsk
L. Ermolaeva
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: angelinapakhomova2011@gmail.com
Россия, Tomsk
V. Krupin
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: angelinapakhomova2011@gmail.com
Россия, Tomsk
N. Ermakova
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: angelinapakhomova2011@gmail.com
Россия, Tomsk
E. Pan
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: angelinapakhomova2011@gmail.com
Россия, Tomsk
A. Kudryashova
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: angelinapakhomova2011@gmail.com
Россия, Tomsk
O. Rybalkina
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: angelinapakhomova2011@gmail.com
Россия, Tomsk
T. Pavlovskaya
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: angelinapakhomova2011@gmail.com
Россия, Tomsk
N. Litvyakov
Tomsk National Research Medical Center, Russian Academy of Sciences
Email: angelinapakhomova2011@gmail.com
Россия, Tomsk
V. Goldberg
Tomsk National Research Medical Center, Russian Academy of Sciences
Email: angelinapakhomova2011@gmail.com
Россия, Tomsk
A. Dygai
E. D. Goldberg Research Institute of Pharmacology and Regenerative Medicine
Email: angelinapakhomova2011@gmail.com
Россия, Tomsk